人骨髓来源多能成体祖细胞ZHJ-MAPCs与人肝细胞系L02体外共培养向肝样细胞的分化
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王海澜, 高 毅, 蒋泽生, 慕 宁
人骨髓来源多能成体祖细胞;肝细胞;直接共培养;间接共培养,王海澜,高毅,蒋泽生,慕宁,王海澜,通讯作者,ZHJ-
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王海澜, 高毅, 蒋泽生, 慕宁, 南方医科大学珠江医院普外科 广东省广州市 510282
王海澜, 1994年哈尔滨医科大学学士, 2004年南方医科大学博士生, 副主任医师, 从事干细胞诱导分化研究.
广东省十五重点科技攻关专项基金资助项目, No. 2002A3020206
通讯作者: 高毅, 510282, 广东省广州, 市南方医科大学珠江医院普外科. gaoyi6146@163.com
电话: 020-61643212
收稿日期: 2006-03-29 接受日期: 2006-04-20
ZHJ-multipotent adult progenitor cells differentiate into hepatocyte-like cells as co-cultured with human liver cell line L02
Hai-Lan Wang, Yi Gao, Ze-Sheng Jiang, Ning Mu
Hai-Lan Wang, Yi Gao, Ze-Sheng Jiang, Ning Mu,Department of General Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China
Supported bythe Key Science and Technology Research Program of the 10th Five-Year Plan of Guangdong Province, No. 2002A3020206
Correspondence to:Dr. Yi Gao, Department of General Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China
Received:2006-03-29 AccePted:2006-04-20
Abstract
AIM: To investigate the feasibility that ZHJ-multipotent adult progenitor cells from bone marrow (ZHJ-MAPCs) differentiate into hepatocytes as co-cultured with human liver cell line L02 in vitro.
METHODS: Co-culture without cell-to-cell contact: ZHJ-MAPCs and L02 hepatocytes were speared on coverslip separately, and then were put in a culture dish. The levels of albumin (ALB), alpha-fetoprotein (AFP), cytokeratin-18 (CK-18), and cytokeratin-19 (CK-19) expression in ZHJ-MAPCs were detected by immunocytochemistry at different time points. Separated culture of L02 hepatocytes served as positive controls and separated culture of ZHJ-MAPCs served as negative controls. Co-culture with cell-to-cell contact: ZHJ-MAPCs labelled with carboxyfluorescein diacetate succidimidyl ester (CFSE) were mixed with L02 hepatocytes (both with cell density of 1×107/L), and then the mixed cells were seeded on special dish for detection by laser scanning confocal microscopy (LSCM) ......
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