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编号:11319739
血管紧张素ⅡⅠ型受体基因A1166C位点基因多态性与肝硬化的关系
http://www.100md.com 黄玉波, 董庆鸣, 宋淑静, 魏红山, 刘志英,
多聚酶链式反应;限制性片段长度多态性分析;纤维化;血管紧张素Ⅱ;血管紧张素Ⅱ受体,黄玉波,董庆鸣,宋淑静,魏红山,刘志英,成军,毛羽
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     黄玉波, 董庆鸣, 宋淑静, 魏红山, 刘志英, 成军, 毛羽, 北京地坛医院 北京市 100011

    北京市优秀人才培养专项经费资助课题(No. 20041D0301548)

    通讯作者:
魏红山, 100011, 北京市东城区安外大街地坛公园13号, 北京地坛医院传染病研究所. drliver@163.com

    电话: 010-64211031-2359

    收稿日期: 2006-05-15 接受日期: 2006-06-08

    A1166C polymorphism of angiotensin Ⅱ type 1 receptor gene and its role in pathogenesis in liver cirrhosis

    
Yu-Bo Huang, Qing-Ming Dong, Shu-Jing Song, Hong-Shan Wei, Zhi-Ying Liu, Jun Cheng, Yu Mao

    Yu-Bo Huang, Qing-Ming Dong, Shu-Jing Song, Hong-Shan Wei, Zhi-Ying Liu, Jun Cheng, Yu Mao,
Beijing Ditan Hospital, Beijing 100011, China

    Correspondence to:
Dr. Hong-Shan Wei, Institute of Infectious Disease, Beijing Ditan Hospital, 13 Ditan Park, Anwai Street, Dongcheng District, Beijing 100011, China. drliver@163.com

    Received:
2006-05-15 Accepted:2006-06-08

    Abstract

    AIM: To establish a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to detect A1166C polymorphism of angiotensin Ⅱ type 1 receptor (AT1R) gene.

    METHODS: A1166C polymorphism was detected and analyzed in 50 anti-HBs positive healthy controls in the serum and 46 patients with liver cirrhosis after hepatitis B virus infection. A set of primers was designed according to AT1R gene. The enzyme digestion site for restriction endonuclease DdeⅠwas also designed around A1166C. A or C allele was assured based on the enzymatic result of the polymerase chain reaction products.

    RESULTS: Sequencing revealed 99% homogeneity between the two homozygous strains and the AT1R gene. The anticipated mutations appeared at position A1166C. As for genotype or allele, there were no significant differences at position A1166C between the hepatitis group and the liver cirrhosis group (P > 0.05).

    CONCLUSION: The established method can be applied to detect the A1166C polymorphism of AT1R gene. The mutation at A1166C site has no correlation with liver fibrosis ......

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