干扰素Ⅱ型；肠道病毒B型_人；疫苗

小鼠IFNγ基因佐剂对分泌型柯萨奇病毒B3 VP1核酸疫苗免疫作用的影响

http://www.100md.com 《第四军医大学学报》 2006年第16期

干扰素Ⅱ型；肠道病毒B型,人；疫苗,DNA；佐剂,免疫；中和抗体；小鼠,,干扰素Ⅱ型；肠道病毒B型，人；疫苗，DNA；佐剂，免疫；中和抗体；小鼠,小鼠IFNγ基因佐剂对分泌型柯萨奇病毒B3VP

     Effect of mouse IFNγ gene adjuvant on immunogenic enhancement of secreting coxsackievirus B3 VP1 DNA vaccine

    CHUAI Xia, GAO ZhiYun, FANG WenLiang, JIN YuHuai, ZHANG YongHong, XIE LiXin, WANG YongXiang

    Department of Pathogenic Biology, School of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China

    【Abstract】 AIM: To construct the eukaryotic expression plasmid pcDNA3/mIFNγ and observe its effect on secreting coxsackievirus B3(CVB3) VP1 gene vaccine. METHODS: mIFNγ cDNA amplified by RTPCR was inserted in eukaryotic expression vector pcDNA3 and then detected after being transfected into cos7 cells with DEAEDextran; Balb/c mice were inoculated in quadriceps muscle at 2week interval for 6 weeks with pcDNA3/sVP1, pcDNA3/sVP1+ pcDNA3/mIFNγ, respectively. The levels of serum antibodies were detected 13 d after each inoculation.The mice were challenged by 500 TCID50 CVB3 2 weeks after the last immunization. RESULTS: Recombinant pcDNA3/mIFNγ plasmid had been constructed and transcripted and expressed. The neutralizing antibody could be induced by pcDNA3/sVP1 and pcDNA3/sVP1+pcDNA3/mIFNγ; The antibody titers increased with the times of inoculation. But there was no difference in the antibody titers and the survival rate between the 2 groups. Coadministration of pcDNA3/mIFNγ and pcDNA3/sVP1 plasmid to Balb/c mice, decreased the titers of virus and reduced the histopathological changes compared with control groups. CONCLUSION: mIFNγ can enhance the immune protection of secreting CVB3 DNA vaccine. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/09/10/16/96/77.htm

您现在查看是摘要页，全文长 11996 字符。