Association of SNPs in proximal promoter region of APM1 gene with serum adiponectin level and type 2 diabetes in Shaanxi Chinese

    HE Lan, LIU Ping, YE Feng , LI Jianª²Ning, DONG Chunª²Ping

    Department of Endocrinology, First Affiliated Hospital, Medical College, Xi¬ðan Jiaotong University, Xi¬ðan 710061, China

    ¡¾Abstract¡¿ AIM: To study the correlation between single nucleotide polymorphisms (SNPs)ª²11377C/G and serum adiponectin level in the Han population with type 2 diabetes in Shaanxi province. METHODS: The PCRª² RFLP techniques were adopted to test SNPs in the proximal promoter region of the APM1 gene in 101 nonª²diabetes control group and 203 diabetes group. The diabetes patients underwent 75 g glucose OGTT and insulin release test for measurement of serum adiponectin, blood sugar, insulin and lipids. Insulin secretion index and insulin resistance index were calculated. RESULTS: Serum adiponectin level in the diabetes group were statistically lower than nonª²diabetes control group [(12.8¡À6.5) mg/L vs (15.0¡À6.9) mg/L, P<0.05]. The distribution of APM1 gene polymorphism in nonª²diabetes group and diabetes group was C/C53(52.5%), C/G38(37.6%), G/G10(9.9%) and C/C107(54.0%), C/G74(37.4%), G/G17(8.6%). In nonª²diabetes control group, serum adiponectin level of the GG genotype at position ª²11377 was lower than that of the CC genotype ,but the TG level of the GG genotype was much higher than that of the CC genotype (P<0.05). CONCLUSION: SNPsª²11377C/G modulate the adipocyteª²secreted adiponectin hormone level and may contribute to the genetic risk for type 2 diabetes in Shaanxi Chinese. ......