【摘要】 目的 构建携带p53基因增殖性腺病毒CNHK600-p53载体，研究其对肝癌细胞株抑制效应是否优于Ad-p53。方法 实验分为两组，单用CNHK600-p53组和单用Ad-p53组；采用四甲基偶氮唑盐(methylthiazolyl tetrazolium assay， MTT)，观察两组对肝癌细胞株的杀伤效应。结果 单用CNHK600-p53组较单用Ad-p53组肝癌细胞株细胞抑制率高；但当细胞抑制率达到80%以上时，两组之间差异无显著性，统计分析采用率的U检验。结论 较Ad-p53相比携带p53基因的CNHK600-p53能更有效抑制肝癌细胞株，CNHK600-p53可望成为肝癌基因治疗的新方法。

    【关键词】 基因；p53； 肝肿瘤； 基因疗法

    The effect of new type replicating adenovirus on the treatment of hepatocarcinoma cell line

    YANG Jia-he, DUAN Ji-cheng, QIAN Qi-jun,et al.

    Department of Comprehensive Treatment Ⅲ，Eastern Hepatobilillary Surgery Hospital，Second Military Medical University，Shanghai 200438,China

    【Abstract】 Objective To construct a replicating adenovirus vector CNHK600-p53 which carried the anti-tumor gene p53,then investigate its inhibition effect is better than Ad-p53 on hepatocarcinoma cell line.Methods The subject is divided into two groups:single CNHK600-p53 group and single Ad-p53 group. The methylthiazolyl tetrazolium assay (MTT)method was used to observe the killing effect of hepatocarcinoma cell line.Results The single CNHK600-p53 group’s inhibition rate is higher than single Ad-p53 group; But when inhibition rate is about to 80%, there are no difference in two groups. The data are analysed by U test.Conclusion Contrast to Ad-p53, adenovirus CNHK600-p53 is more effective in inhibition to hepatocarcinoma cell line. CNHK600-p53 may be an new gene method for hepatocarcinoma. ......