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肝癌组织中A2b腺苷受体的表达及其意义
http://www.100md.com 《第四军医大学学报》 2006年第14期
肝肿瘤;受体,腺苷A2b;聚合酶链反应,,肝肿瘤;受体,腺苷A2b;聚合酶链反应,【摘要】,【关键词】,1材料和方法,2结果,3讨论,【参考文献】
     XIANG HongJun, DOU KeFeng, WANG DeSheng, YANG YanLing, LIU ZhengCai, CHEN Yong

    Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】AIM: To assess whether A2b adenosine receptor is differentially expressed in malignant tissues and evaluate A2b adenosine receptor level in hepatocellular carcinoma(HCC). METHODS: We determined the expression of A2b adenosine receptor protein by immunohistochemistry and Western blot and evaluated the expression levels of A2b adenosine receptor mRNA by using realtime quantitative PCR analysis in surgical samples of 38 HCC cases and in their paired adjacent noncancerous tissues. RESULTS: Immunohistochemistry showed a high expression of A2b adenosine receptor in human HCC. The levels of A2b adenosine receptor mRNA by realtime PCR in HCC were significantly higher than those in adjacent tissues (P<0.001). Weston blot analysis revealed the intensity of A2b adenosine receptor protein expression was constantly much higher in HCC than that in adjacent tissues normalized to βactin levels in an arbitrary unit. CONCLUSION: A2b adenosine receptor expression appeared upregulated in HCC, and it might be a new target molecule for HCC therapeutic strategy. ......

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