T细胞激活剂(期刊论文)

T细胞激活剂对Jurkat 细胞重组激活基因表达的影响

http://www.100md.com 《第四军医大学学报》 2006年第14期

T细胞激活剂,；Jurkat细胞；重组激活基因；逆转录聚合酶链反应,,T细胞激活剂,；Jurkat细胞；重组激活基因；逆转录聚合酶链反应,T细胞激活剂对Jurkat细胞重组激活基因表达的影响,【

     ZOU HongYun1, MA Li1, YAO XinSheng1, WEN Qian1, WANG XiaoNing2

    1Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China, 2School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510641, China

    【Abstract】 AIM: To investigate the effects of T cell activators on the mRNA expression of recombinationactivating genes (RAGs) in Jurkat cells. METHODS: After Jurkat cells were treated with PHA (20 mg/L), antiCD3 mAb (1∶100), PMA(40 μg/L)+A23187 (0.5 μmol/L) for 6 and 12 h respectively, RAG1 and RAG2 mRNA were measured by RTPCR. Semiquantitative analysis was used to evaluate RAG1 and RAG2 mRNA levels in different groups. RESULTS: Compared with the control groups, the levels of RAG1 mRNA were significantly lower in different treated groups (P<0.05), and RAG1 mRNA levels were associated with treatment time. RAG2 mRNA levels in PHA treated groups were significantly lower than those in the control groups (P<0.05). No RAG2 mRNA was detected in PMA+A23187 treated groups. However, RAG2 mRNA level was significantly higher in antiCD3 mAb (12 h) treated group than that in control groups(P<0.05). CONCLUSION： RAG1 and RAG2 mRNA were coexpressed in Jurkat cells and could be regulated by T cell activators. The results may give a clue that Jurkat cells maybe provide an ideal cell line model for studying the regulation of RAGs in peripheral T cells. ......

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