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编号:11177047
胎鼠听囊细胞体外培养研究
http://www.100md.com 《第四军医大学学报》 2006年第14期
听囊细胞;干细胞;细胞增殖;细胞分化;胎鼠,,听囊细胞;干细胞;细胞增殖;细胞分化;胎鼠,【摘要】,【关键词】,1材料和方法,2结果,3讨论,【参考文献】
     LIU Hui1, CHENG SuiTao2, XU Min1, LIANG JianMin1, MA WeiJun1

    1Department of Otolaryngology, Second Hospital, Xian Jiaotong University, Xian 710004,China, 2Department of Five Senses, Shaanxi Corps Hospital, Chinese Peoples Armed Police Forces, Xian 710054, China

    【Abstract】 AIM: To find the origin of auditory cells in mammals and to study the proliferation and differentiation of auditory cell progenitors. METHODS: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells. RESULTS: Otocyst cells differentiated and proliferated and showed several morphotypes, mainly including epitheliallike cells, fibroblastlike and neuronlike cells. All of cells were labeled positive to Factin and cytokeratin. A subset of cells were labeled strong positive by calretinin in primary culture. But few passaged cells were positive to calretinin. BrdU labeling showed cultured cells could proliferate significantly. The morphological phenotype was retained throughout 8 passages during 4month culture. CONCLUSION: In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells. The study will provide some ideas and methods for further research about hair cell regeneration. ......

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