大鼠实验性肝癌发生中卵圆细胞的变化
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朱言亮, 陈孝平, 张万广, 裘法祖
肝干细胞;卵圆细胞;肝癌;P53;实验大鼠,朱言亮,陈孝平,张万广,裘法祖,朱言亮,通讯作者:,Roleofovalcellsincarcinogenesi
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朱言亮, 陈孝平, 张万广, 裘法祖, 华中科技大学同济医学院附属同济医院肝脏外科 湖北省武汉市 430030
朱言亮, 1996年蚌埠医学院本科毕业, 2006年华中科技大学同济医学院博士, 主要从事肝癌的基础研究.
国家自然科学基金资助课题, No. 30430670
通讯作者: 朱言亮, 430030, 湖北省武汉市, 华中科技大学同济医学院附属同济医院肝脏外科. yl_zhu@126.com
收稿日期: 2006-04-09 接受日期: 2006-04-30
Role of oval cells in carcinogenesis of experimental hepatocellular carcinoma in rats
Yan-Liang Zhu, Xiao-Ping Chen, Wan-Guang Zhang, Fa-Zu Qiu
Yan-Liang Zhu, Xiao-Ping Chen, Wan-Guang Zhang, Fa-Zu Qiu,Center of Hepatic Surgery, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Supported byNational Natural Science Foundation of China, No. 30430670
Correspondence to:Yan-Liang Zhu, Center of Hepatic Surgery, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. yl_zhu@126.com
Received:2006-04-09 Accepted:2006-04-30
Abstract
AIM: To discuss the role of oval cells in the carcinogenesis and progression of hepatocellular carcinoma.
METHODS: A total of 60 male Sprague Dawley rats were divided into experiment group (n = 48) and control group (n = 12). 3’-methyl-4-dimethylaminoazobenzene (3’-Me-DAB) was used to induce hepatocellular carcinoma, and the presence of oval cells, the expression of p53 gene and P53 protein were dynamically (4, 8, 12, 16, 20, 24 wk) detected by immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively.
RESULTS: Large numbers of oval cells appeared in the periportal regions 4 wk after the induction of carcinoma, and these cells were OV-6-positive. Cancer nodules were observed at the 16th wk, and the oval cells were located in and around the cancer nodule. A part of these proliferated cells were P53-positive, and the two kinds of cells were almost located in the same region ......
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