EGCG对人胃癌细胞株MKN 45中Bcl-2家族相关基因表达的影响
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童锦禄, 冉志华, 陈 翔, 萧树东
表没食子儿茶素没食子酸酯;细胞凋亡;胃癌;Bcl-2童锦禄,冉志华,陈翔,萧树东.EGCG对人胃癌细胞株MKN45中Bcl-2家族相关基因表达的影响. 世界华人消化杂志2006;14(30
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童锦禄, 冉志华, 陈翔, 萧树东, 上海交通大学医学院附属仁济医院、上海市消化疾病研究所 上海市 200001
童锦禄, 上海交通大学医学院(原上海第二医科大学)硕士, 现攻读博士学位, 主要从事消化道肿瘤的基础研究.
上海市重点学科建设资助项目, No. Y0205
通讯作者: 冉志华, 200001, 上海市, 上海交通大学医学院附属仁济医院、上海市消化疾病研究所. z-ran@online.sh.cn
电话: 021-63287817 传真: 021-63266027
收稿日期: 2006-08-23 接受日期: 2006-09-21
Expression of the Bcl-2 family is influenced by Epigallocatechin-3 -gallate in human gastric cancer cell MKN 45
Jin-Lu Tong, Zhi-Hua Ran, Xiang Chen, Shu-Dong Xiao
Jin-Lu Tong, Zhi-Hua Ran, Xiang Chen, Shu-Dong Xiao, Renji Hospital, School of Medicine, Shanghai Jiao Tong University; Shanghai Institute of Digestive Diseases, Shanghai 200001, China
Supported byShanghai Leading Academic Discipline Project, No. Y0205
Correspondence to: Zhi-Hua RAN, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Diseases, Shanghai 200001, China. z-ran@online.sh.cn
Received: 2006-08-23 Accepted: 2006-09-21
Abstract
AIM:To investigate the changes of the Bcl-2 family in human gastric cancer cell line MKN 45 after epigallocatechin-3-gallate (EGCG) treatment.
METHODS: The mRNA expression of apoptosis-related genes Bcl-xl, Bcl-2, Bax, Bid and Bad were examined by reverse transcription-polymerase chain reaction (RT-PCR). Western blot analysis was performed to detect the changes of the corresponding protein expression, and then the ratio of Bcl-2 to Bax (Bcl-2/Bax) was calculated.
RESULTS: The mRNA and protein expression levels of pro-apoptotic members, such as Bax, Bid and Bad, were increased gradually as the treating time extended (Bax protein: 0.6292 ± 0.08, 0.6454 ± 0.04, 0.8069 ± 0.09, 0.8992 ± 0.07, P < 0.05; Bid protein: 1.1214 ± 0.08, 1.3697 ± 0.07, 1.610 ± 0.09, 2.0623 ± 0.12, P < 0.05; Bad protein: 0 ......
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