肝细胞癌变过程中胰岛素样生长因子Ⅱ的动态表达与改变特征
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邱历伟, 姚登福, 宗 蕾, 吴 玮, 吴信华
肝癌;胰岛素样生长因子Ⅱ;免疫组化;酶联免疫吸附试验邱历伟,姚登福,宗蕾,吴玮,吴信华.肝细胞癌变过程中胰岛素样生长因子-Ⅱ的动态表达与改变特征. 世界华人消化杂志2006;14(30)2
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邱历伟, 姚登福, 宗蕾, 吴玮, 吴信华, 南通大学附属医院临床分子生物学中心 江苏省南通市 226001
邱历伟, 南通大学肿瘤学硕士生, 主要从事肿瘤早期诊断及分子机制研究. 江苏省卫生厅项目, No. H200523
南通市社会发展科技计划, No. S5053
通讯作者: 姚登福, 226001, 江苏省南通市, 南通大学附属医院临床分子生物学中心. yaodf@ahnmc.com
电话: 0513-85052523
收稿日期: 2006-08-16 接受日期: 2006-09-16
Dynamic expression and alteration characteristic of insulin-like growth factor-Ⅱ in the carcinogenesis of hepatocellular carcinoma
Li-Wei Qiu, Deng-Fu Yao, Lei Zong, Wei Wu, Xin-Hua Wu
Li-wei Qiu, Deng-Fu Yao, Lei Zong, Wei Wu, Xin-Hua Wu, Research Center of Clinical Molecular Biology, the Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China
Supported by Fund from Health Department of Jiangsu Province, No. H200523, and Nantong Social Development Project of Science and Technology, No. S5053
Correspondence to: Professor Deng-Fu Yao, Research Center of Clinical Molecular Biology, the Affiliated Hospital of Nantong University, Nantong 226001, Province, China. yaodf@ahnmc.com
Received: 2006-08-16 Accepted: 2006-09-16
Abstract
AIM:To investigate the dynamic expression and alteration of insulin-like growth factor-Ⅱ(IGF-Ⅱ) during in the pathogenesis and development of hepatocellular carcinoma.
METHODS: Hepatoma models were induced with 2-fluorenylacetamide (2-FAA) in male Sprague-Dawley (SD) rats. The morphological changes of rat livers were observed by HE staining. The dynamic changes of IGF-Ⅱ in rat livers and sera were quantitatively analyzed by enzyme linked immunosorbent assay (ELISA). Simultaneously, the expression and cellular distribution of IGF-Ⅱ were detected by immunohistochemistry.
RESULTS: Histological examination confirmed that the expression of IGF-Ⅱwas increased in gradient after granule-like degeneration, atypical hyperplasia and carcinoma appeared in hepatocytes, and it was significantly higher in carcinoma group than that in degeneration and control group (carcinoma vs degeneration, c2 = 9 ......
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