Egr-1基因表达及其在射线诱导的肝癌细胞凋亡中的作用
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董广璐, 邢丽娜, 刘晓滨, 刘 伟, 金 茜,
X射线;Egr-1基因;细胞凋亡;细胞周期;肝癌细胞系董广璐,邢丽娜,刘晓滨,刘伟,金茜,张淑云.Egr-1基因表达及其在射线诱导的肝癌细胞凋亡中的作用. 世界华人消化杂志2006;14(
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董广璐, 邢丽娜, 刘晓滨, 哈尔滨医科大学附属第二医院肿瘤放射治疗科 黑龙江省哈尔滨市 150086
刘伟, 金茜, 张淑云, 哈尔滨医科大学附属第二医院科研实验中心 黑龙江省哈尔滨市 150086
董广璐, 1993年哈尔滨医科大学硕士, 副主任医师, 副教授, 主要从事肿瘤放射治疗.黑龙江省科技计划攻关项目, No. GC03C606-4
通讯作者: 张淑云, 150086, 哈尔滨市南岗区学府路246号, 哈尔滨医科大学附属第二医院科研实验中心.
zhshuyun136@yahoo.com.cn
电话: 0451-86605487 传真: 0451-86688011
收稿日期: 2006-09-04 接受日期: 2006-10-12
Early growth response-1 gene expression and its role in radiation-induced apoptosis of liver cancer cell lines
Guang-Lu Dong, Li-Na Xing, Xiao-Bin Liu, Wei Liu, Qian Jin, Shu-Yun Zhang
Guang-Lu Dong, Li-Na Xing, Xiao-Bin Liu, Department of Tumor Radiotherapy, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Wei Liu, Qian Jin, Shu-Yun Zhang, Research Center, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Supported by the Key Science and Technology Project of Heilongjiang Province, No. GC03C606-4
Correspondence to: Shu-Yun Zhang, Research Center, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China. zhshuyun136@yahoo.com.cn
Received: 2006-09-04 Accepted: 2006-10-12
Abstract
AIM:To study the relationship between radiation-induced apoptosis and the expression of early growth response-1 (Egr-1) gene in liver cancer cell lines.
METHODS:The cultured cells (HepG2, SMMC-7721 and HL-7702) were irradiated at 4Gy X-radiation. The expression of Egr-1 gene was detected by fluorescent quantitative-polymerase chain reaction (FQ-PCR) before and 1, 2, 4, 6, 12, 24 h after irradiation. Cell cycle and apoptosis were detected by flow cytometry (FCM).
RESULTS:The expression of Egr-1 was increased from 1 to 4 h after irradiation in the three kinds of cell lines HepG2, SMMC-7721 and HL-7702, and the highest expression was in HepG2 cells (DEgrHepG2 = 12 ......
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