eNOS基因转染对慢性缺氧性肺动脉高压的影响
高血压,肺性;基因疗法;一氧化氮合酶;重组,遗传;腺病毒科,,高血压,肺性;基因疗法;一氧化氮合酶;重组,遗传;腺病毒科,0引言,1材料和方法,2结
Effect of adenovirusmediated human endothelial nitric oxide synthase gene transfer on chronic hypoxic pulmonary hypertensionZHOU Jin, WANG JunKe, ZHANG TieZheng, JIN Qiang, ZHANG YiNan, WANG FengXue
Department of Anesthesiology, First Affiliated Hospital, China Medical University, Shenyang 110001, China, Department of Anesthesiology, General Hospital of Shenyang Military Area Command, Shenyang 110016, China
【Abstract】 AIM: To investigate the effect of adenovirusmediated human endothelial nitric oxide synthase (eNOS) gene(AdCMVceNOS) transfered to the rat lungs through airway on chronic hypoxic pulmonary hypertension(CHPH) rats and to explore its possible role in CHPH therapy. METHODS: Thirty male Wistar rats were randomly divided into 3 groups(n=10 per group). the control group(group C), the chronic hypoxic group(group H), the chronic hypoxic and AdCMVceNOS transferred group(group T). The AdCMVceNOS (3×109 pfu) were tranfered into the rat lungs through the respiratory tract.Survey the pulmonary artery pressure(PAP), right ventricular weight and content of NO in pulmonary artery respectively after 3 d. The expression activity of eNOS was observed by immunohistochemistry and Western blot methods. RESULTS: The mean PAP and the RV/(LV+S) of group H and T increased obviously compared with group C (P<0.05); The mean PAP of group T decreased compared with group H (P<0.05); Compared with group C,the content of NO in group H decreased obviously (P<0.05), but group T was increased more obviously than group H (P<0.05). The A value of eNOS in group H and T increased obviously compared with that of group C (P<0.05), but the group T was significantly different from group H in A value (P<0.05). The levels of eNOS protein in group H (0.62±0.08) and T(0.92±0.17)were much higher than that in group C (0.30 ±0.05). CONCLUSION: Gene transfer of eNOS to the lung may be a useful therapeutic intervention for the treatment of CHPH, which may be correlated with the increased eNOS activity and NO content. ......
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