脑缺血再灌注；_一氧化氮合酶；_核转录因子_

大鼠急性脑缺血再灌注后NOS、NFκB的表达及意义

http://www.100md.com 《新疆医科大学学报》 2006年第8期

脑缺血再灌注；,一氧化氮合酶；,核转录因子,,脑缺血再灌注；,一氧化氮合酶；,核转录因子,1材料与方法,2结果,3讨论,参考文献:

     摘要: 目的：研究大鼠急性脑缺血再灌注(I/R)后不同时间段脑组织不同部位一氧化氮合酶(NOS)、核转录因子(NFκB)的表达及意义。方法：采用PulsineuiBrierley法建立大鼠I/R模型。72只大鼠随机分为对照组、缺血30 min组(I组)、缺血30 min再灌注后0、2、4、6、8、10、12 h组，于在脑组织臂旁核、室旁核、杏仁核、蓝斑所在部位作冠状切片，分别进行HE和免疫组化染色，光镜下观察各组脑组织的病理改变，并比较各组大鼠脑组织不同部位的表达。结果：脑I/R 2 h组脑组织神经细胞肿胀，少量炎性细胞浸润。I/R 8 h组脑组织神经细胞核碎裂、消失，间隙增宽，结构疏松，神经细胞局部溶解、液化性坏死范围较广。与对照组相比，其余各组脑组织不同部位NFκB的表达均明显上调，差异有统计学意义(P均<0.05)，I/R 2、4、6、8、10、12 h组NOS表达明显上调，差异有统计学意义(P<0.05)。脑缺血再灌注损伤诱导NOS表达于8 h时达高峰，8～12 h呈下降趋势。NFkB表达于0~8 h呈上升趋势，8~12 h较稳定。结论：NOS、NFkB在脑组织损伤过程中发挥调控作用，一氧化氮(NO)在调控中起双重作用。

    关键词: 脑缺血再灌注；一氧化氮合酶；核转录因子

    The expression and singnificance of NOS and NFκB following

    acute brain ischemia reperfusion in ratsWU Xiuzhen, HU Xia, HOU Hui

    (Department of Medical Neurology, Fifth Affiliated Hospital, Xinjiang Medical University,

    Urumqi 830011, China)Abstract: Objective: To discuss the adjust mechinism of nitric oxide synthase(NOS) and NFkappaB (NFΚB) in brain and in different time following brain ischemia reperfusion. Methods: A classical animal model of brain ischemiareperfusion was established by imitation. Seventytwo healthy rats were randomly divided into 9 groups: control group, ischemia 30 min, reperfusion immediatoly after ischemia 30 min, 2 hr reperfusion after ischemia 30 min, 4 hr reperfusion after ischemia 30 min, 6 hr reperfusion after ischemia 30 min, 8 hr reperfusion after ischemia 30 min, 10 h reperfusion after ischemia 30 min, 12 hr reperfusion after ischemia 30 min. The varying of expressing of NOS, NFκB in different time and different brain tissue including amygdaloid nucleus, paraventricular nucleus, nucleus ceruleus were examined by immune chemical technique. Results: Among 8~10 h group a apart of nueral cell have karyolysis and liquefactive necrosis. Expressing of NOS have apprant ascend trend in I/R0~8 h group. The expression of NOS reached the higest in I/R8 h. The expression of NOS have statistic significance among the I/R0 h, I/R2 h, I/R4 h, I/R6 h, I/R8 h, I/R10 h, and I/R12 h groups. The expression of NFκB had statistic significance among I/R0 h, I/R2 h, I/R4 h, I/R6 h, I/R8 h, I/R4 h, I/R6 h, and I/R8 h groups. Expressing of NFκB have apprant ascend trend in I/R08 h group. Conclusions: NOS and NFκB may intervene in the chains of the cell injures in ischemiareperfusion injure of brain. amygdaloid nucleus, paraventricular nucleus, nucleus ceruleus may participate in regulative effect of central nerve systeme. NO had double effect. ......

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