肝门阻断再灌注后脑皮层组织和血清S100b蛋白的差异性改变及意义
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郑晓春, 陈卫民, 盖成林
肝门阻断;再灌注损伤;S100β蛋白;脑损伤;Wistar大鼠,郑晓春,陈卫民,郑晓春,盖成林,通讯作者:,Alterat
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郑晓春, 陈卫民, 中国医科大学附属盛京医院麻醉科 辽宁省沈阳市 110001
郑晓春, 福建省立医院麻醉科 福建省福州市 350001
盖成林, 中国人民解放军第230医院麻醉科 辽宁省丹东市 118001
通讯作者: 陈卫民, 110001, 沈阳市和平区三好街36号, 中国医科大学附属盛京医院麻醉科. chenwm@cmu2h.com
电话: 024-83955029 传真: 024-83955029
收稿日期: 2006-05-13 接受日期: 2006-07-10
Alteration of cortex and serum S100β protein in rats undergoing hepatic portal occlusion reperfusion
Xiao-Chun Zheng, Wei-Min Chen, Cheng-Lin Gai
Xiao-Chun Zheng, Wei-Min Chen,Department of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Xiao-Chun Zheng,Department of Anesthesiology, Fujian Provincial Hospital, Fuzhou 350001, Fujian Province, China
Cheng-Lin Gai,Department of Anesthesiology, the 230th Hospital Chinese PLA, Dandong 118001, Liaoning Province, China
Correspondence to:Wei-Min Chen, Department of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China. chenwm@cmu2h.com
Received:2006-05-13 Accepted:2006-07-10
Abstract
AIM: To explore the alterations of neuroglia cell reactivity and central nervous system (CNS) injury in rats undergoing hepatic portal occlusion (HPO) reperfusion using S100β protein tissue content and serum concentration respectively.
METHODS: A total of 38 Wistar rats were anesthetized by isoflurane inhalation. HPO model was produced by hepatic portal interruption (Pringle' maneuver) for 30 min with a clamp, followed by 6-, 12-, and 24-h reperfusion after removal of the clamp. The animals were randomly divided into group A (n = 8) and B (n = 30). Group A served as the sham operation group, while HPO model was induced in group B. The pH values, Ca2+ and K+ concentrations of portal vein blood sample were measured by blood-gas analysis, and the brain cortex content and serum concentration of S100β protein were measured by enzyme-linked immunosorbent assay (ELISA) ......
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