外源质粒DNA对小鼠肠道基因表达谱的影响
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刘建文, 乐国伟, 施用晖
外源质粒DNA;肠道;基因表达;寡核苷酸芯片,刘建文,乐国伟,施用晖,刘建文,通讯作者:,EffectofforeignplasmidDNAongen
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刘建文, 乐国伟, 施用晖, 教育部食品科学与安全重点实验室 江南大学食品营养与安全研究所 江苏省无锡市 214036
刘建文, 分子营养学博士, 主要从事分子营养与代谢调控研究, 现任希杰(中国)有限公司技术总监.
国家自然科学基金资助项目, No. 30270970
通讯作者: 刘建文, 200336, 上海虹桥开发区兴义路8号万都中心610室. ljw_ftpt@yahoo.com.cn
电话: 021-52082925 传真: 021-52080290
收稿日期: 2005-03-22 接受日期: 2005-10-09
Effect of foreign plasmid DNA on gene expression profile of the intestine in mice
Jian-Wen Liu, Guo-Wei Le, Yong-Hui Shi
Jian-Wen Liu, Guo-Wei Le, Yong-Hui Shi,the Key Laboratory of Food Science and Safety , Ministry of Education, Institute of Food Nutrition and Safety, Southern Yangtze University, Wuxi 214036, Jiangsu Province, China
Supported byNational Natural Science Foundation of China, No. 30270970
Correspondence to:Dr. Jian-Wen Liu, Room 610, Maxdo Centre, 8 Xingyi Road, Hongqiao Development Zone, Shanghai 200336, China. ljw_ftpt@yahoo.com.cn
Received:2005-03-22 AccePted:2005-10-09
Abstract
AIM: To explore the influence of foreign plasmid DNA on the intestinal gene expression profile after absorption via the gastrointestinal tract in mice.
METHODS: Balb/c mice were orally administered with 200 μg plasmid pcDNA3 and a length of jejunum was isolated 4 h after oral administration. The total RNA was extracted from the intestine and the gene expression profile of Balb/c mice was analyzed using oligonucleotide array after oral administration of plasmid pcDNA3.
RESULTS: Among 17667 genes obtained from gene expression profile analysis, there were 61 genes differentially expressed from those in GenBank, of which 36 genes were up-regulated and 25 ones were down-regulated after oral administration of foreign plasmid DNA. The differentially expressed genes were mainly related to immune response, anti-oxidation, detoxification function, lipid metabolism, anion transport protein, apoptosis and signal transduction.
CONCLUSION: Foreign plasmid DNA, administered via the gastrointestinal tract, may widely modulate the expression of many genes in the intestine in mice ......
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