肝癌组织内类阿片肽LVV-血衍吗啡素-6的作用
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周 迈, 彭吉润, 钟朝辉, 涂向群, 常迎彬,
血衍吗啡素;肝细胞癌;组织特异性肽池;细胞凋亡,周迈,涂向群,常迎彬,焦岗军,钟朝辉,彭吉润,冷希圣,周迈,通讯作者:,Roleofopio
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周迈, 涂向群, 常迎彬, 焦岗军, 民航总医院普外科 北京市 100025
钟朝辉, 彭吉润, 冷希圣, 北京大学人民医院肝胆外科 北京市 100044
周迈, 2005北京大学第二临床医学院研究生毕业, 医学博士, 主治医师, 主要从事肝癌免疫治疗的研究.
国家自然基金资助项目, No. 3020071
通讯作者: 周迈, 100025, 北京市朝阳区高井甲1号, 民航总医院普外科. zhoumai109@yahoo.com.cn
电话: 010-85762244-2382
收稿日期: 2006-06-15 接受日期: 2006-08-14
Role of opioid peptide Leu-Val-Val-hemorphin-6 in hepatocellular carcinoma
Mai Zhou, Ji-Run Peng, Zhao-Hui Zhong, Xiang-Qun Tu, Ying-Bin Chang, Gang-Jun Jiao, Xi-Sheng Leng
Mai Zhou, Xiang-Qun Tu, Ying-Bin Chang, Gang-Jun Jiao,Department of General Surgery, General Hospital of Civil Aviation Administration of China, Beijing 100025, China
Zhao-Hui Zhong, Ji-Run Peng, Xi-Sheng Leng,Department of Hepatobiliary Surgery, People’s Hospital of Peking University, Beijing 100044, China
Supported byNational Natural Science Foundation of China, No. 3020071
Correspondence to:Dr. Mai Zhou, Department of General Surgery, General Hospital of Civil Aviation Administration of China, 1 Gaojingjia, Chaoyang District, Beijing 100025, China. zhoumai109@yahoo.com.cn
Received:2006-06-15 Accepted:2006-08-14
Abstract
AIM: To assay the content differences of hemorphins between hepatocellular carcinoma (HCC) and normal liver tissues, and to explore the role of hemorphins in HCC.
METHODS: The tissue samples were obtained from 12 patients with HCC, and peptides were isolated by mild acid elution from the surfaces of hepatocarcinoma and normal hepatic cells. The mixtures of peptides were analyzed by high performance liquid chromatography (HPLC) and mass spectrometer (MS). A differential comparison was made on the fractions of peptides between HCC and normal liver cells to screen some tumor-specific peptides. Finally, human hepatocarcinoma cell line HLE was treated with different concentrations of the obtained peptides (0, 10-7, 10-8, 10-9, 10-10, 10-11, 10-12 mol/L), and the growth and apoptosis of HLE cells were detected by MTT assay and flow cytometry (FCM), respectively ......
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