【摘要】 目的 研究酒精致大鼠学习记忆障碍与基底前脑内侧隔核胆碱能神经元变化的关系。方法 将24只雄性Wister大鼠随机均分成对照组和酒精中毒组，酒精中毒组大鼠隔日胃内注入含酒精(2.5g/kg)的蒸馏水2ml共90d，对照组则注入不含酒精的蒸馏水2ml。然后采用Morris水迷宫和免疫组织化学技术，分析大鼠基底前脑内侧隔核胆碱能神经元、海马星形胶质细胞数的变化和学习记忆能力的关系。结果 酒精中毒组大鼠Morris水迷宫潜伏期明显延长，基底前脑内侧隔核胆碱能神经元数目明显减少，海马星形胶质细胞数目反应性增生。结论 酒精致大鼠学习记忆能力障碍与基底前脑内侧隔核胆碱能神经元减少有关。

    【关键词】 酒精 学习记忆 胆碱能神经元 星形胶质细胞 内侧隔核

    Changes of Learning Memory and of Cholinergic Neurons of Medial Septum Induced by Chronic Alcoholism in Rats

    Cui Weigang,et al.

    (Department of Anatomy,Xinxiang medical College,Xinxiang 453000)

    【Abstract】 Objective To investigate the effect of chronic alcoholism on the learnig memory as well as on the Cholinergic Neurons of medial septum of adult rats. Methods Healthy adult Wistar rats (n=24) were randomly divided into the following groups: normal control group,chronic alcoholism group(alcohol was given (2.5g/kg) intragastrically by gavages once every other day for 90 days). Immunohistochemical analyses were made on choline acetytransferase (ChAT )and glial fibrillary acidic protein(GFAP).The abilities of learning and memory were detected in Morris water maze. Results The number of choline acetytransferase immunoreactive (ChAT-IR) cells in medial septum and the abilities of learning memory were significantly decreased in chronic alcoholism group(P<0.01).Conclusions Alcohol can cause malfunction on learning memory ......