【摘要】 目的 用重组表达的gp210融合蛋白检测gp210抗体，以利于原发性胆汁性肝硬化PBC的早期诊断。方法 采用重组表达的gp210融合蛋白，建立了免疫印迹法(IBT)和酶联免疫吸附试验(ELISA)。检测50例原发性胆汁性肝硬化患者血清的gp210抗体，以及其他肝病患者、自身免疫病患者、健康体检者作对照。结果 50例PBC患者血清中检测出抗gp210抗体阳性21例，阴性为29例，阳性率为42%，而疾病对照组和健康体检者血清中该抗体检测均为阴性。结论 用重组表达的人gp210融合蛋白检测抗体，有较好的敏感性和特异性，有助于PBC的临床诊断。

    【关键词】 gp210； 肝硬化；胆汁性；自身抗体；免疫印迹；酶联免疫吸附试验

    Detection of anti-gp210 autoantibody using recombinant gp210 and the clinical significance

    ZHOU Ye,CHEN Yan, JIANG Tian-shu, et al.Laboratory Diagnostics, Changzheng Hospital,Second Military Medical University,and Clinical Immunology Center of PLA, Shanghai 200003, China

    【Abstract】 Objective To detect gp210 autoantibody using recombinant gp210 fusion protein in order to help diagnosis of primary biliary cirrhosis(PBC). Methods Using recombinant gp210, we established immune blotting test(IBT) and enzyme linked immunosorbent assays (ELlSA) to detect gp210 autoantibody in the sera of patients with primary biliary cirrhosis(PBC), compared with groups of healthy, other liver diseases and other autoimmune diseases.Results Among 50 sera from PBC patients, 21 were positive,while all controls were negative. The positive rate was 42%.Conclusion The recombinant gp210 could be used to detect gp210 autoantibody specially and sensitively, helpful for diagnosis of PBC. ......