WANG Chengª²Bin1, HUANG Zhenª²Guo2, YE Weiª²Ji2, LI Luoª²Yi2, BAO Yiª²Xi2, TONG Hongª²Li3, TIAN Yaª²Ping3, LIN Weiª²Ji2

    1Department of Clinical Laboratory Medicine, 3Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853, China, 2Department of Chemical Pathology, Chinese University of Hong Kong, Hong Kong, China

    ¡¾Abstract¡¿ AIM: To investigate the effect of different culture systems on ILª²6 release in model of coª²culture of human bronchial epithelial cells and eosinophils. METHODS: Human bronchial epithelial cells and eosinophils were cultured or coª²cultured in separation, contact and Matrigel system. ILª²6 release in different culture systems of bronchial epithelial cells and eosinophils was determined by enzymeª²linked immunosorbent assay (ELISA). RESULTS: Compared with the sum of ILª²6 in culture supernatants of bronchial epithelial cells and eosinophils alone, ILª²6 release exhibited a synergistic effects in coª²culture of the two kinds of cells in a well separated into up and down chambers by the screen, that could sustain the culture supernatant across [(107¡À10) ng/L vs (388¡À40) ng/L, P<0.001]. But ILª²6 release in coª²culture of the two kinds of cells separated using screen was less than that in their contact coª²culture [(388¡À40) ng/L vs (1270¡À131) ng/L, P<0.001)]. Coª²culture of bronchial epithelial cells and eosinophils in Matrigel system led to asignificant elevation of ILª²6 release compared with that in general contact coª²culture of the two kinds of cells [(2076¡À165) ng/L vs (1270¡À131) ng/L, P<0.01)]. CONCLUSION: Human bronchial epithelial cells and eosinophils can be activated to release ILª²6 by contacting each other and mediators that they released in coª²culture. ......