[摘要] [目的] 制备重组人干扰素α2a凝胶剂，并研究部分药效和毒理学. [方法] 以羧甲基纤维素钠为基质制备重组人干扰素凝胶剂，利用FL传代细胞检测凝胶在体外培养单纯疱疹病毒(HSV)的抑制作用，并进行家兔体内局部用药毒性试验及稳定性试验. [结果] 所制备重组人干扰素α2a凝胶剂的稳定性良好，局部用药未见皮肤刺激反应及急性毒性反应，对HSV-Ⅱ的抑制效果优于对HSV-Ⅰ. [结论] 所制备重组人干扰素α2a具有制备工艺简单、稳定性好及无毒副作用等特点，且对HSV有抑制作用.

    [关键词] 干扰素α，重组;凝胶;药物，评估

    ZHANG Shu-zi 1 ，ZHANG Jing-lan 2 ，DONG Yi-lan 3

    (1.Jilin Yatai Biopharmaceuticals Co.，LTD.，Changchun130033，Jilin，China;2.Department of Chemotherapy，Tumor Hospital of Jilin Province，Changchun130021，Jilin，China;3.Changchun Changsheng Gene Pharmaceutical Co.，LTD.Changchun130012，Jilin，China)

    ABSTRACT:OBJECTIVE To prepare rhIFNα2a gel and study on its effectiveness and toxicology.METHODS CMC-Na was used as the gel base，the pharmacological effects of anti-HSV in vitro was studied with FL cell ......