[摘要] [目的] 建立人免疫球蛋白转基因小鼠实验性自身免疫性重症肌无力模型. [方法] 用电鳐电器官分离纯化的乙酰胆碱受体免疫人免疫球蛋白转基因小鼠，以肌肉收缩力判定病情，以放射免疫测定法测定动物血清中抗乙酰胆碱受体抗体滴度及动物全身肌肉乙酰胆碱受体浓度. [结果] 实验动物血清中抗乙酰胆碱受体抗体滴度为6～296nmol，全身肌肉乙酰胆碱受体损失率最高达65%，部分动物出现肌肉收缩无力表现. [结论] 成功地建立了人免疫球蛋白转基因小鼠实验性自身免疫性重症肌无力模型.

    [关键词] 重症肌无力;模型;小鼠

    Institution of model of myasthenia gravis in transgenic mice

    LI Hong-hua 1 ，JIN Gui-hua 1 ，MENG Fan-ping 1* ，LI Ying-xin 1 ，LI Fang-fang 1 ，SUN Chang-yuan 1 ，JIN Quan-xin 1 ，Stassen M 2 ，de Baets M 2

    (1.Department of Immunology and Pathogenic Biology，Yanbian University College of Basic Medicine，Yanji133000，Jilin，China;2.Department of Neurology，Faculty of Medicine，University of Maastricht，Maastricht，the Netherlands)

    ABSTRACT:OBJECTIVE To set up a new model of experimental autoimmune myasthenia gravis(EAMG)in mice transgenic for human immunoglobulin(Ig)loci.METHODS Mice transgenic for human Ig were immunized with acetylcholine receptor(AChR) ......