肌酸激酶同工酶MB(CKMB)；荧光免疫测定_

CKMB时间分辨荧光免疫分析的建立

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肌酸激酶同工酶MB(CKMB)；荧光免疫测定,,肌酸激酶同工酶MB(CKMB)；荧光免疫测定,1材料和方法,2结果,3讨论,【参考文献】

     Timeresolved fluoroimmunoassay of CKMB

    HANG JianFeng, WU YingSong, XU WeiWen, YU WeiHong, LI Ming

    School of Biotechnology, Southern Medical University, Guangzhou 510515 China, Guangzhou Darui Antibody CO. LTD, Guangzhou 510663, China

    【Abstract】 AIM: To make a fast quantitative detection of Creatine KinaseMB Isoenzyme(CKMB) based on timeresolved fluoroimmunoassay(TRFIA). METHODS: The sandwich TRFIA was established for detection of serum CKMB level using antiCKMB mAbs(One monoclonal antibody was immobilized on surface of microtiter plate strip wells, and another was labelled with Eu3+). RESULTS: The linear measurement range of CKMB TRFIA was 5-400 μg/L. The sensitivity was 0.24 μg/L. The intra and interassay coefficients of variation(CV) were 4.9%-7.3%and 8.4%-11.7%, respectively. There was no crossreaction to CKMM and CKBB. The correlation coefficient of 82 blood samples detected by CKMB TRFIA and commercially available CLIA kit was 0.895. CONCLUSION: CKMB TRFIA is a new immunoassay which has several advantages including high sensitivity, specificity and widely working range. It is suitable for routine clinical detection of serum CKMB level. ......

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