组织工程；_支架材料；_β磷酸三钙多孔陶瓷_

beagle犬骨髓间质干细胞与β磷酸三钙多孔陶瓷复合培养的实验研究

http://www.100md.com 《新疆医科大学学报》 2006年第11期

组织工程；,支架材料；,β磷酸三钙多孔陶瓷,,组织工程；,支架材料；,β磷酸三钙多孔陶瓷,1材料与方法,2结果,3讨论

     摘要: 目的：研究beagle犬骨髓间质干细胞(BMSCs)与β磷酸三钙多孔陶瓷(βTCP)的生物相容性。方法：体外诱导培养beagle 犬BMSCs作为种子细胞，βTCP作为组织工程支架，制备βTCPBMSCs复合物，相差倒置显微镜及扫描电镜观察细胞在材料孔隙区的贴附及生长情况。结果：相差倒置显微镜观察：自原代、2代、3代及4代培养，贴壁细胞由成纤维细胞样向三角形、棱形、多边形和多角形转化，最终重叠生长形成结节状；将细胞接种于材料上培养2~4 d，孔隙间大量生长增殖，呈细胞交叠覆盖。扫描电镜下观察：材料表面细胞呈梭形，相互融合成片状，表面有丝状突起，孔隙边缘有大量胶原分泌及细胞外基质分泌。结论：βTCP与细胞的亲和力好，能够为基质细胞增殖、分化和成骨提供自然的三维空间，是极具潜力的组织工程支架之一。

    关键词：组织工程；支架材料； β磷酸三钙多孔陶瓷

    Preparation of the compound of osteoblast and βporous calcium phosphate ceramics

    HE Huiyu, Adalaiti Ahemaitijiang, LI Jing, et al

    (Department of Stomatology, First Affilicated Hospital, Xinjiang Medical University,Urumqi 830054, China)

    Abstract: Objective: To study the biocompatibility between the BMSCs of beagle with βTCP. Methods: Through vitro inducement cultivation, the BMSCs of Beagles was used as seed cell. βTCP as the tissue engineering bracket, the βTCP with BMSCs complexion was made. To observe the cell storage and growing condition on material pore with scanning electric microscope and phase contract inversion electronic microscope. Results: According to observation from the phase contrast inversion electric microscope primary cultivate from passage Ⅰ to passage Ⅳ, adherent dependcell convert into fibroblastlike. Triangle diamond and polygon ultimately formation nodosity through overlap growing cell will be into material. Cultivated for 4 days cells generated dramatically. In the material pore showing the coincide growing observing through scanning electronic microscope fingershaped cell from the material surface confluent lamella. The surface had filamentous protrusion. The bargain of pore had a plenty of collagen and extracellular matrix secret. Conclusion: βTCP has good affinity with adherent dependcell and can be providing natural the 3dimesional space for matrix cell generation ossification differentation. βTCP is the one of the potential development for the tissue engineering brand. The βporous calcium phosphate ceramics is one of the most potential scaffold materials of tissue engineering. ......

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