高胆固醇血症兔血氧饱和度及相关病理生理学改变
高胆固醇血症;,血气分析;,细胞低氧;,氧化应激;,血氧测定法;,兔,,高胆固醇血症;,血气分析;,细胞低氧;,氧化应激;,血氧测定法;,兔,1材料
摘要: 目的 观察兔高胆固醇摄入后血氧饱和度及相关病理生理学改变,探讨高胆固醇血症(HC)早期微循环损害及其机制。 方法 新西兰兔分2组:对照组和HC组,前者喂饲普遍饲料，后者喂饲高胆固醇饲料，3周后检测血清总胆固醇(TC)含量、静注乙酰胆碱(ACh)前后动静脉血血气分析及血压、肾组织血管通透性、胸主动脉环ACh介导的舒张反应、脑匀浆一氧化氮合酶(NOS)和超氧化物歧化酶(SOD)的活力及一氧化氮(NO)和丙二醛(MDA)的含量。 结果 HC组血清TC较对照组显著升高(P<0.01);静注ACh后对照组静脉血血氧饱和度(SO2)显著降低(P<0.05),HC组降低不明显且降血压效应明显减弱(P<0.05);与对照组比较,HC组肾组织血管通透性明显增加(P<0.01);ACh介导的胸主动脉环舒张功能降低;脑组织NOS活力及MDA含量均显著增加(P<0.05),NO生成量显著减少(P<0.01)。 结论 HC早期出现SO2等血气分析指标异常,提示HC可能妨碍血氧向组织的弥散过程,导致组织细胞摄氧减少,发生慢性或间歇性缺氧,从而引起或加剧氧化应激产生损害。关键词: 高胆固醇血症; 血气分析; 细胞低氧; 氧化应激; 血氧测定法; 兔
Blood Saturation of Oxygen and Interrelated Pathophysiology
in Rabbits with Hypercholesterolemia
Wang Huiying, Wei Wenshu
Department of Pharmacy, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, China
ABSTRACT: Objective To study the early damaging mechanism of hypercholesterolemia(HC) in blood saturation of oxygen(SO2) and interrelated pathophysiology in rabbits. Methods The rabbits were randomly divided into two groups: normal control(NC) group and HC group(fed with high cholesterol feed for 3 weeks). At the end of the treatment, total serum cholesterol(TC), SO2 and other blood gas analysis indexes of arterial blood and venous blood, artery blood pressure, renal capillary permeability, acetylcholine(ACh)induced endotheliumdependent relaxation of thoracic aortic ring, the content of nitric oxide(NO) and malondiadehyde(MDA), and the activity of nitric oxide synthase(NOS) and superoxide dismutase(SOD) in cerebral tissue were determined, respectively, before and after iv ACh. Results The serum TC was significantly increased in HC group(P<0.01).The SO2 of venous blood was significantly decreased and BP reduced. After ACh iv in control group, but no significant change in HC group. Compared with NC group, the renal capillary permeability was increased in HC group. AChinduced endotheliumdependent relaxation of thoracic aortic and the hypotensive effect of ACh iv was significantly diminished in HC group.The NOS activity and MDA content in cerebral tissue were increased, and the NO content was decreased in HC group. Conclusion That the decrease in the SO2 of venous blood in HC rabbits. That HC may impair the diffusion of oxygen from blood to tissue, which lead to the chronic hypoxia. ......
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