糖尿病大鼠肾皮质细胞过度凋亡
糖尿病肾病;,增殖细胞核抗原;,凋亡;,大鼠,,糖尿病肾病;,增殖细胞核抗原;,凋亡;,大鼠,1材料和方法,2结果,3讨论,参考文献:
摘要: 目的 研究糖尿病大鼠肾皮质细胞凋亡的可能机制及其对糖尿病肾病的影响。 方法20只雄性SD大鼠随机分为糖尿病组和对照组。糖尿病大鼠模型采用链脲佐菌素65 mg/kg腹腔注射诱导建立。12周后观察2组体质量、血糖以及血肌酐、尿素氮、24 h尿蛋白等肾功能指标;采用透射电镜和原位末端标记法(TUNEL法)检测大鼠肾皮质细胞凋亡,并用免疫组织化学的方法检测增殖细胞核抗原(PCNA)以及凋亡相关基因Bax、Bcl2、Caspase3的表达。 结果建模12周的糖尿病组肾小球和肾小管细胞凋亡明显增多(P<0.01),肾小球和肾小管细胞PCNA表达无明显差别(P>0.05);与对照组比较,糖尿病组肾小管Bax和Caspase3表达明显增加(P<0.01),Bcl2表达明显减少(P<0.05);2组肾小球都不表达Bax和Bcl2,糖尿病组肾小球有少量Caspase3表达。 结论 糖尿病大鼠肾皮质细胞过度凋亡。包括Bax、Bcl2和Caspase3在内的Caspase依赖的线粒体凋亡途径可能参与了糖尿病状态下肾小管细胞的凋亡;细胞凋亡的增多可能是糖尿病肾病发展的原因之一。关键词: 糖尿病肾病; 增殖细胞核抗原; 凋亡; 大鼠
Excessive Apoptosis in Diabetic Rat Renal Cortex
Jin Jiansheng, Su Bofeng, Ye Hong
Departmeat of Nephrology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, China
ABSTRACT: Objective To investigate the mechanisms of cell apoptosis in diabetic rat renal cortex and the effects of apoptosis on the development of diabetic nephropathy. Methods Twenty male SpragueDawley rats were randomly assigned to two groups: diabetic model group and control group. Diabetic rats were induced by intraperitoneal injection of streptozotocin(65 mg/kg). After 12 weeks, the body weight, serum glucose, serum creatinine, blood urea nitrogen and 24hour urine protein excretion were checked. Apoptotic cells in renal cortex were checked by transmission electron microscope and terminal deoxynucleotidyl transferasemediated dUDP nickend labeling(TUNEL). The expression of PCNA, Bax, Bcl2 and Caspase3 was examined by immunohistochemistry. ResultsDiabetes caused significantly increase in apoptosis in glomerular and tubular cells in renal cortex(P<0.01). Compared with those in normal control group, the levels of Bax and Caspase3 expression increased remarkably(P<0.01), and the level of Bcl2 decreased significantly(P<0.05) in renal cortical tubules in diabetic group. No expressions of Bax and Bcl2 were detected in the glomerular cells of both groups. Caspase3 expressed slightly increased in the glomeruli of diabetic group. Conclusion Compared with the degree of cell proliferation, the cell apoptosis was relatively increased in the renal cortex of diabetic rats at 12th week. Activation of the Caspasedepedent apoptotic pathway by increasing the expression of Bax, Caspase3 and decreasing the expression of Bcl2 may contribute to apoptosis in renal cortical tubular cells of diabetic rats. Excessive apoptosis may play an important role in the development of diabetic nephropathy. ......
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