益气活血软坚解毒方含药血清诱导人肝癌细胞系Bel-7402细胞凋亡过程中部分凋亡调控基因变化
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李东涛, 孙桂芝, 裴迎霞, 祁 鑫, 李 杰, 李富玉, 李军艳, 许彦来,
益气活血软坚解毒方;含药血清;肝癌;细胞凋亡;基因调控,李东涛,李富玉,李军艳,许彦来,陈美南,孙桂芝,裴迎霞,祁鑫,李杰,李东涛,通讯作者:,电话:,收稿日期:,接受日期:,YiqiHu
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李东涛, 李富玉, 李军艳, 许彦来, 陈美南, 济南军区青岛第一疗养院中医科 山东省青岛市 266071
孙桂芝, 裴迎霞, 祁鑫, 李杰, 中国中医科学院广安门医院肿瘤科 北京市 100053
李东涛, 2003年中国中医科学院医学博士, 博士后, 副主任医师, 主要研究方向中医肿瘤及中医老年病的基础与临床研究工作.
国家科技计划资助项目, No. C03050402
通讯作者: 李东涛, 266071, 山东省青岛市香港西路27号, 济南军区青岛第一疗养院中医科. lidongtao12345@126.com
电话: 0532-83785529
收稿日期: 2006-06-09 接受日期: 2006-10-11
Yiqi Huoxue Ruanjian Jiedu decoction regulates expression and activity of apoptosis-regulated genes in human hepatocellular carcinoma cell line BEL-7402
Yu Wang, Li-Guang Sun, Chun-Hui Xia
Dong-Tao Li, Fu-Yu Li, Jun-Yan Li, Yan-Lai Xu, Mei-Nan Chen, Department of Traditional Chinese Medicine, the First Sanatorium of Ji’nan Military Command, Qingdao 266071, Shandong Province, China
Gui-Zhi Sun, Ying-Xia Pei, Xin Qi, Jie Li, Department of Oncology, Guang’anmen Hospital, China Institute of Traditional Chinese Medicine, Beijing 100052, China
Correspondence to: Dong-Tao Li, Department of Traditional Chinese Medicine, the First Sanatorium of Ji’nan Military Command, Qingdao 266071, Shandong Province, China. lidongtao12345@126.com
Received: 2006-06-09 Accepted: 2006-10-11
AbstractAIM:To investigate the effects of Yiqi Huoxue Ruanjian Jiedu (YHRJ) decoction on the expression of apoptosis-related genes Fas, FasL, Bcl-2, Bax, P53 and NF-kB in human hepatocellular carcinoma cell line BEL-7402.
METHODS: Human hepatocellular carcinoma BEL-7402 cells were divided into 6 groups, and treated with normal saline (NS), NS plus cisplatin (DDP), equal-dose YHRJ decoction, YHRJ decoction plus DDP group, high-dose YHRJ decoction, high-dose YHRJ decoction plus DDP, respectively. Flow cytometry, immunohistochemistry, in situ hybridization, and reverse transcription-polymerase chain reaction (RT-PCR) were used to analyze the expression of Fas, FasL, Bcl-2, Bax, P53 and NF-kB.
RESULTS: Flow cytometry revealed a dramatic increase of Fas expression in the cells treated with NS plus DDP, equal-dose YHRJ and equal-dose YHRJ plus DDP, and high-dose YHRJ plus DDP in comparison with that in the control cells (30 ......
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