Delving deeper into MALT lymphoma biology
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《临床调查学报》
Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland, Bellinzona, Switzerland.
Abstract
Mucosa-associated lymphoid tissue (MALT) lymphomas can arise in a variety of extranodal sites. Interestingly, at least 3 different, apparently site-specific, chromosomal translocations, all affecting the NF-B pathway, have been implicated in the development and progression of MALT lymphoma. The most common is the translocation t(11;18)(q21;q21), which results in a fusion of the cIAP2 region on chromosome 11q21 with the MALT1 gene on chromosome 18q21 and is present in more than one-third of cases. The frequency of this translocation is site-related: common in the gastrointestinal tract and lung, rare in conjunctiva and orbit, and almost absent in salivary glands, thyroid, liver, and skin. In this issue of the JCI, Hu et al. add to our understanding of the molecular consequences of this translocation, showing that its fusion product, cIAP2-MALT1, may concomitantly contribute to lymphomagenesis both as a tumor suppressor gene and as an oncogene.
See the related article beginning on page 174
MALT lymphoma: the clinical background
Extranodal marginal zone B cell lymphoma (EMZL) of mucosa-associated lymphoid tissue (MALT), also known as MALT lymphoma, is a neoplastic disease that constitutes approximately 8% of all non-Hodgkin lymphomas (1-4). It can arise in a variety of extranodal sites and occurs most often in organs such as the stomach, the salivary glands, or the thyroid, where lymphocytes are normally absent. MALT is the result of chronic phlogistic events in response to either infectious conditions such as Helicobacter pylori–associated chronic gastritis, or autoimmune disorders like Hashimoto thyroiditis and myoepithelial sialadenitis. In these conditions abnormal B cell clones can progressively replace the normal B cell population of the inflammatory tissue, giving rise to the EMZL. Over 10 years ago, H. pylori was identified as an etiologic factor in gastric MALT lymphomas after the demonstration of tumor regression in the majority of early-stage cases treated with anti-Helicobacter antibiotic therapy, and this tumor therefore became a popular model of the close pathogenetic link between chronic inflammation and lymphoma development. Other bacterial infections were later possibly implicated in the pathogenesis of MALT lymphomas arising in the skin (Borrelia burgdorferi), in the ocular adnexa (Chlamydia psittaci), and in the small intestine (Campylobacter jejuni) (1, 4).
The prognosis for patients with MALT lymphomas is good; these tumors usually have an indolent course with overall 5-year survival rates greater than 80%, but rare cases with histologic transformation in cases of aggressive diffuse large-cell lymphoma have been described. It is nowadays generally accepted that eradication of H. pylori with antibiotics should be used as the sole initial treatment of localized gastric MALT lymphoma, while the use of anti-infectious treatment in nongastric locations is still under investigation. Other effective treatment approaches include radiotherapy, chemotherapy, and anti-CD20 mAbs (2, 3).
Many chromosomal translocations affecting the same pathway
Four main recurrent chromosomal translocations have been associated with the pathogenesis of EMZLs: t(11;18)(q21;q21), t(1;14)(p22;q32), t(14;18)(q32;q21), and t(3;14)(p14.1;q32) (5-8) (Table 1). The latter is the most recently described and establishes the juxtaposition of the transcription factor FOXP1 next to the enhancer region of the Ig heavy chain genes (8); the pathogenetic relevance of this translocation is still unclear. Interestingly, the other 3 seemingly disparate translocation types appear to affect the same signaling pathway, resulting in the activation of NF-B, a transcription factor with a central role in immunity, inflammation, and apoptosis (1). The t(1;14)(p22;q32) translocation is detected in only 1–2% of cases of EMZL. The translocation results in overexpression of the BCL10 gene due to the juxtaposition with the promoter region of the Ig heavy chain genes. The BCL10 gene (also known as CARMEN, CIPER, CLAP, c-E10, and mE10) codes for an adaptor protein implicated, as discussed below, in surface receptor signaling and highly expressed in the nucleus of the neoplastic B cells of EMZLs carrying this translocation (6, 9). The t(14;18)(q32;q21) translocation, described in approximately 20% of EMZL cases and cytogenetically identical to the t(14;18)(q32;q21) involving BCL2 in follicular lymphoma, juxtaposes the MALT1 gene (also known as MLT and MLT1) next to the promoter region of the Ig heavy chain genes with subsequent MALT1 overexpression (7). The t(11;18)(q21;q21) translocation is the most common translocation, occurring in 15–40% of cases (1, 4, 5, 10, 11). It results in the reciprocal fusion of cellular inhibitor of apoptosis protein 2 (cIAP2; also known as AIP1, API2, BIRC3, HAIP1, HIAP1, MALT2, and MIHC) on 11q21 with MALT1 on 18q21. The creation of a fusion protein encoded by cIAP2-MALT1 on the derivative chromosome 11 is the pathogenetic event.
The main players: cIAP2, MALT1, and BCL10
The cIAP2 protein belongs to the inhibitor of apoptosis protein (IAP) family, characterized by the presence of 1–3 baculoviral IAP repeat (BIR) domains (12-15). cIAP2 contains 3 N-terminal BIRs, a middle caspase recruitment domain (CARD), and a C-terminal zinc-binding RING finger domain (Figure 1A). MALT1, a paracaspase, comprises an N-terminal death domain (DD), followed by 2 Ig-like C2 domains, and a caspase-like domain (Figure 1B) (14-16). All the breakpoints in the cIAP2 gene occur downstream of the third BIR domain but upstream of the C-terminal RING domain, with over 90% of the breaks occurring just before the CARD (Figure 1A) (1, 2, 4). Conversely, the breakpoints in MALT1 are variable but always upstream of the caspase-like domain (Figure 1B) (1, 2, 4). Thus, the resulting fusion protein always comprises the N-terminal region of cIAP2, with 3 intact BIR domains, and the C-terminal MALT1 region, containing an intact caspase-like domain (Figure 1C). The specific conservation of certain functional domains of cIAP2 and MALT1 to form a fusion product strongly suggests the importance and synergy of these domains in oncogenic activities. NF-B activation is one of the main downstream effects of the stimulation of cell-surface receptors, such as B cell or T cell receptors. In unstimulated cells, NF-B molecules are sequestered in the cytoplasm, because of the binding with inhibitory B (IB) proteins. The IB protein is phosphorylated by the IB kinase (IKK) heterodimer. The phosphorylation leads to ubiquitylation and degradation of IB; NF-B can migrate to the nucleus and act as transcription factor. The IKK complex comprises 2 catalytically active kinases (IKK and IKK) and a regulatory component (IKK, also known as NEMO). Both MALT1 and BCL10, 2 of the genes involved in the above-mentioned translocations, are known to be upstream of the IKK complex (14-19). BCL10 binds to CARMA1 (also known as CARD11 and BIMP3) and to MALT1. The BCL10, CARMA1, and MALT1 complex activates NF-B via IKK degradation (14-16, 18, 19). MALT1 binds to BCL10 at the Ig-like domains, and to CARMA1 at the caspase-like domain. The t(11;18)(q21;q21) fusion protein cIAP2-MALT1 is an activator of NF-B, and it bears a gain of function when compared with the WT MALT1 (20, 21).
Novel insights into cIAP2 function
The work presented by Hu et al. in this issue of the JCI (22) addresses a very important open question: the role of cIAP2 in normal mammalian cells and in the context of the fusion protein. Since the RING domain of IAPs can have a ubiquitin ligase (E3) activity, which determines the specificity of the ubiquitylation process, and since the latter is fundamental in protein degradation (Figure 2) (13), Hu and colleagues hypothesized that cIAP2 might also be part of the antigen receptor signaling pathway. First, RNA and protein levels of BCL10 in mammalian cells were assessed in a series of t(11;18)(q21;q21)-positive and -negative cases. Indeed, protein and not RNA levels were higher in MALT lymphoma cases bearing the chromosomal translocation, suggesting a stabilization of the protein. The possible interaction between BCL10 and cIAP2 was then investigated by immunoprecipitation both in transfected human embryonic kidney 293T cells and in untransfected primary human T cells. BCL10 and cIAP2 clearly associated with each other in both experimental models. In particular, the association was increased after T cell activation and with the phosphorylated form of BCL10. By transfection of cIAP2 deletion mutants, BCL10 was shown to bind to the BIR region of cIAP2 and not to the RING domain or CARD. As is known for other IAPs (13), Hu et al. showed that the RING domain of cIAP2 has ubiquitin ligase (E3) activity (22). Because of the BIR-mediated binding, cIAP2 induced BCL10 ubiquitylation (Figure 3A). In T lymphocytes, BCL10 degradation occurred after stimulation with anti–CD3/CD28 antibodies, but not after treatment of the cells with TNF-, indicating that the degradation was specific to antigen receptor stimulation. The BCL10 degradation was dependent on cIAP2: in fact, transfection of primary T cells with WT cIAP2 induced greater BCL10 degradation, while transfection with cIAP2 mutants missing the RING domain determined upregulation of BCL10 and blocked BCL10 degradation after cell stimulation. These data indicate the highly relevant role that cIAP2 plays in regulating BCL10 response after antigen receptor stimulation. As mentioned above, the cIAP2-MALT1 fusion protein created by the t(11;18)(q21;q21) chromosomal translocation always lacks the RING domain of cIAP2, while always containing the BIR domains (1, 2, 4). Indeed, Hu and colleagues demonstrated that cIAP2-MALT1 is able to bind BCL10 but not able to ubiquitylate it (Figure 3B) (22). Interestingly, BCL10 and cIAP2-MALT1 synergistically activated NF-B, and, at least in HeLa cells, the downregulation of endogenous BCL10 caused a decreased response to NF-B activation induced by expression of the cIAP2-MALT1 fusion protein. These data indicate that BCL10 expression might be needed for cIAP2-MALT1 function. As a whole, the Hu et al. study (22) shows that cIAP2 as well as MALT1 and BCL10 is involved in physiologic antigen receptor stimulation. The EMZL-specific cIAP2-MALT1 fusion gene would behave both as a tumor suppressor gene, since it is no longer able to downregulate BCL10 expression, and also as an oncogene, since the fusion protein induces strong NF-B activation (22). These data are in perfect accordance with the high expression levels of BCL10 observed in clinical EMZL cases bearing the t(11;18)(q21;q21) translocation (9). The BCL10 protein is expressed only in the cytoplasm in non-neoplastic germinal center and marginal zone B cells as well as in EMZLs with neither t(1;14)(p22;q32) nor t(11;18)(q21;q21) translocations (9). Conversely, nuclear expression is present both in the t(1;14)-positive and in the t(11;18)-positive EMZLs, suggesting that nuclear localization of BCL10 can occur as the result of 2 apparently independent cytogenetic events. While the significance of this change in cellular localization is not yet known, a possible partial explanation has recently been presented by Nakagawa et al. (23), albeit in partial disagreement with the data reported here by Hu et al. (22). Because of the presence of 2 nuclear export signal (NES) domains (Figure 1A), MALT1 would export BCL10 from the nucleus to the cytoplasm (23). In the presence of the t(1;14)(p22;q32) chromosomal translocation, the overexpression of BCL10 would lead to increased BCL10 nuclear compartmentalization (13). In the presence of the t(14;18)(q32;q21) translocation, with increased WT MALT1 expression, the localization of BCL10 would remain cytoplasmic, as observed in normal B cells (23, 24). In the presence of the t(11;18)(q21;q21) translocation, the effect on BCL10 cellular localization is still unclear. While in the study reported by Nakagawa et al. (23) the cIAP2-MALT1 fusion protein has lost the ability to bind BCL10, possibly explaining the accumulation of BCL10 in the nucleus, according to Hu and colleagues (22) this protein would retain its ability to bind BCL10. Since the constructs used in the 2 studies are relatively comparable, differences in the cell systems in which the experiments have been carried out could at least partially explain the differences in the observed results. However, further studies of the significance of nuclear BCL10 expression are mandatory.
Outlook
Another field where additional studies will be welcome is the role played by antigens and by the concomitant chronic phlogistic status in the pathogenesis of EMZL. Indeed, EMZLs are considered, at least partially, to be antigen dependent. In addition to epidemiological and therapeutic evidence, another basis for this belief was the demonstration that EMZL cells would often recognize self-antigens (25, 26). However, a very recent publication by Lenze et al. (27) reported new data disproving a direct role for the Igs expressed by EMZLs in sustaining lymphoma growth. The fact that only 1 out of 7 lymphomas expressed specific Igs (actually, directed against a plasma cell protein) makes the direct influence of antigen or self-antigen much less important in sustaining the growth of lymphoma cells. Indeed, in gastric MALT lymphoma, H. pylori–specific T cells are necessary to sustain the in vitro growth of lymphoma cells (28). Considering also the extreme importance of non-neoplastic cells in the pathogenesis and the definition of prognosis of follicular lymphomas (another indolent B cell lymphoma) (29, 30), further investigation of nonlymphoma cells in EMZL appears worthwhile.
The 4 recurrent chromosomal translocations demonstrate a site-specificity in terms of their incidence (10). Indeed, another open question that remains unaddressed is the possible effect of infectious agents in inducing individual translocations and how lymphoma risk depends on the microenvironmental effects of the interaction between genetic factors of the microorganisms (11) and the genetic background of the host (31). Nevertheless, if bacterial and host factors have a relevant impact on the initiation of the lymphomagenesis process, the role of the genetic damage eventually acquired by the tumor lymphocytes is important as well. In this context, the data provided by Hu et al. (22) shed light on the molecular consequences of the most common translocation in MALT lymphoma, showing that the t(11;18)(q21;q21) fusion product (cIAP2-MALT1) may concomitantly contribute to the lymphomagenesis, acting either as a tumor suppressor gene (loss of function) or as an oncogene (gain of function). Indeed, as a result of the cIAP2-MALT1 fusion, cIAP2’s capacity for ubiquitylation and degradation of BCL10 is lost, and NF-B appears to be synergistically activated by BCL10 (which is no longer degraded) and cIAP2-MALT1. The combination of both properties of cIAP2-MALT1 appears crucial in the strong NF-B activation observed in lymphocytes harboring the t(11;18)(q21;q21) translocation, but the relative contribution to the oncogenic potential of these 2 properties of cIAP2-MALT1 remains to be determined.
Footnotes
Nonstandard abbreviations used: BIR, baculoviral IAP repeat; CARD, caspase recruitment domain; cIAP2, cellular inhibitor of apoptosis protein 2; DD, death domain; EMZL, extranodal marginal zone B cell lymphoma; IAP, inhibitor of apoptosis protein; IB, inhibitory B; IKK, IB kinase; MALT, mucosa-associated lymphoid tissue; NES, nuclear export signal.
Conflict of interest: The authors have declared that no conflict of interest exists.
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Abstract
Mucosa-associated lymphoid tissue (MALT) lymphomas can arise in a variety of extranodal sites. Interestingly, at least 3 different, apparently site-specific, chromosomal translocations, all affecting the NF-B pathway, have been implicated in the development and progression of MALT lymphoma. The most common is the translocation t(11;18)(q21;q21), which results in a fusion of the cIAP2 region on chromosome 11q21 with the MALT1 gene on chromosome 18q21 and is present in more than one-third of cases. The frequency of this translocation is site-related: common in the gastrointestinal tract and lung, rare in conjunctiva and orbit, and almost absent in salivary glands, thyroid, liver, and skin. In this issue of the JCI, Hu et al. add to our understanding of the molecular consequences of this translocation, showing that its fusion product, cIAP2-MALT1, may concomitantly contribute to lymphomagenesis both as a tumor suppressor gene and as an oncogene.
See the related article beginning on page 174
MALT lymphoma: the clinical background
Extranodal marginal zone B cell lymphoma (EMZL) of mucosa-associated lymphoid tissue (MALT), also known as MALT lymphoma, is a neoplastic disease that constitutes approximately 8% of all non-Hodgkin lymphomas (1-4). It can arise in a variety of extranodal sites and occurs most often in organs such as the stomach, the salivary glands, or the thyroid, where lymphocytes are normally absent. MALT is the result of chronic phlogistic events in response to either infectious conditions such as Helicobacter pylori–associated chronic gastritis, or autoimmune disorders like Hashimoto thyroiditis and myoepithelial sialadenitis. In these conditions abnormal B cell clones can progressively replace the normal B cell population of the inflammatory tissue, giving rise to the EMZL. Over 10 years ago, H. pylori was identified as an etiologic factor in gastric MALT lymphomas after the demonstration of tumor regression in the majority of early-stage cases treated with anti-Helicobacter antibiotic therapy, and this tumor therefore became a popular model of the close pathogenetic link between chronic inflammation and lymphoma development. Other bacterial infections were later possibly implicated in the pathogenesis of MALT lymphomas arising in the skin (Borrelia burgdorferi), in the ocular adnexa (Chlamydia psittaci), and in the small intestine (Campylobacter jejuni) (1, 4).
The prognosis for patients with MALT lymphomas is good; these tumors usually have an indolent course with overall 5-year survival rates greater than 80%, but rare cases with histologic transformation in cases of aggressive diffuse large-cell lymphoma have been described. It is nowadays generally accepted that eradication of H. pylori with antibiotics should be used as the sole initial treatment of localized gastric MALT lymphoma, while the use of anti-infectious treatment in nongastric locations is still under investigation. Other effective treatment approaches include radiotherapy, chemotherapy, and anti-CD20 mAbs (2, 3).
Many chromosomal translocations affecting the same pathway
Four main recurrent chromosomal translocations have been associated with the pathogenesis of EMZLs: t(11;18)(q21;q21), t(1;14)(p22;q32), t(14;18)(q32;q21), and t(3;14)(p14.1;q32) (5-8) (Table 1). The latter is the most recently described and establishes the juxtaposition of the transcription factor FOXP1 next to the enhancer region of the Ig heavy chain genes (8); the pathogenetic relevance of this translocation is still unclear. Interestingly, the other 3 seemingly disparate translocation types appear to affect the same signaling pathway, resulting in the activation of NF-B, a transcription factor with a central role in immunity, inflammation, and apoptosis (1). The t(1;14)(p22;q32) translocation is detected in only 1–2% of cases of EMZL. The translocation results in overexpression of the BCL10 gene due to the juxtaposition with the promoter region of the Ig heavy chain genes. The BCL10 gene (also known as CARMEN, CIPER, CLAP, c-E10, and mE10) codes for an adaptor protein implicated, as discussed below, in surface receptor signaling and highly expressed in the nucleus of the neoplastic B cells of EMZLs carrying this translocation (6, 9). The t(14;18)(q32;q21) translocation, described in approximately 20% of EMZL cases and cytogenetically identical to the t(14;18)(q32;q21) involving BCL2 in follicular lymphoma, juxtaposes the MALT1 gene (also known as MLT and MLT1) next to the promoter region of the Ig heavy chain genes with subsequent MALT1 overexpression (7). The t(11;18)(q21;q21) translocation is the most common translocation, occurring in 15–40% of cases (1, 4, 5, 10, 11). It results in the reciprocal fusion of cellular inhibitor of apoptosis protein 2 (cIAP2; also known as AIP1, API2, BIRC3, HAIP1, HIAP1, MALT2, and MIHC) on 11q21 with MALT1 on 18q21. The creation of a fusion protein encoded by cIAP2-MALT1 on the derivative chromosome 11 is the pathogenetic event.
The main players: cIAP2, MALT1, and BCL10
The cIAP2 protein belongs to the inhibitor of apoptosis protein (IAP) family, characterized by the presence of 1–3 baculoviral IAP repeat (BIR) domains (12-15). cIAP2 contains 3 N-terminal BIRs, a middle caspase recruitment domain (CARD), and a C-terminal zinc-binding RING finger domain (Figure 1A). MALT1, a paracaspase, comprises an N-terminal death domain (DD), followed by 2 Ig-like C2 domains, and a caspase-like domain (Figure 1B) (14-16). All the breakpoints in the cIAP2 gene occur downstream of the third BIR domain but upstream of the C-terminal RING domain, with over 90% of the breaks occurring just before the CARD (Figure 1A) (1, 2, 4). Conversely, the breakpoints in MALT1 are variable but always upstream of the caspase-like domain (Figure 1B) (1, 2, 4). Thus, the resulting fusion protein always comprises the N-terminal region of cIAP2, with 3 intact BIR domains, and the C-terminal MALT1 region, containing an intact caspase-like domain (Figure 1C). The specific conservation of certain functional domains of cIAP2 and MALT1 to form a fusion product strongly suggests the importance and synergy of these domains in oncogenic activities. NF-B activation is one of the main downstream effects of the stimulation of cell-surface receptors, such as B cell or T cell receptors. In unstimulated cells, NF-B molecules are sequestered in the cytoplasm, because of the binding with inhibitory B (IB) proteins. The IB protein is phosphorylated by the IB kinase (IKK) heterodimer. The phosphorylation leads to ubiquitylation and degradation of IB; NF-B can migrate to the nucleus and act as transcription factor. The IKK complex comprises 2 catalytically active kinases (IKK and IKK) and a regulatory component (IKK, also known as NEMO). Both MALT1 and BCL10, 2 of the genes involved in the above-mentioned translocations, are known to be upstream of the IKK complex (14-19). BCL10 binds to CARMA1 (also known as CARD11 and BIMP3) and to MALT1. The BCL10, CARMA1, and MALT1 complex activates NF-B via IKK degradation (14-16, 18, 19). MALT1 binds to BCL10 at the Ig-like domains, and to CARMA1 at the caspase-like domain. The t(11;18)(q21;q21) fusion protein cIAP2-MALT1 is an activator of NF-B, and it bears a gain of function when compared with the WT MALT1 (20, 21).
Novel insights into cIAP2 function
The work presented by Hu et al. in this issue of the JCI (22) addresses a very important open question: the role of cIAP2 in normal mammalian cells and in the context of the fusion protein. Since the RING domain of IAPs can have a ubiquitin ligase (E3) activity, which determines the specificity of the ubiquitylation process, and since the latter is fundamental in protein degradation (Figure 2) (13), Hu and colleagues hypothesized that cIAP2 might also be part of the antigen receptor signaling pathway. First, RNA and protein levels of BCL10 in mammalian cells were assessed in a series of t(11;18)(q21;q21)-positive and -negative cases. Indeed, protein and not RNA levels were higher in MALT lymphoma cases bearing the chromosomal translocation, suggesting a stabilization of the protein. The possible interaction between BCL10 and cIAP2 was then investigated by immunoprecipitation both in transfected human embryonic kidney 293T cells and in untransfected primary human T cells. BCL10 and cIAP2 clearly associated with each other in both experimental models. In particular, the association was increased after T cell activation and with the phosphorylated form of BCL10. By transfection of cIAP2 deletion mutants, BCL10 was shown to bind to the BIR region of cIAP2 and not to the RING domain or CARD. As is known for other IAPs (13), Hu et al. showed that the RING domain of cIAP2 has ubiquitin ligase (E3) activity (22). Because of the BIR-mediated binding, cIAP2 induced BCL10 ubiquitylation (Figure 3A). In T lymphocytes, BCL10 degradation occurred after stimulation with anti–CD3/CD28 antibodies, but not after treatment of the cells with TNF-, indicating that the degradation was specific to antigen receptor stimulation. The BCL10 degradation was dependent on cIAP2: in fact, transfection of primary T cells with WT cIAP2 induced greater BCL10 degradation, while transfection with cIAP2 mutants missing the RING domain determined upregulation of BCL10 and blocked BCL10 degradation after cell stimulation. These data indicate the highly relevant role that cIAP2 plays in regulating BCL10 response after antigen receptor stimulation. As mentioned above, the cIAP2-MALT1 fusion protein created by the t(11;18)(q21;q21) chromosomal translocation always lacks the RING domain of cIAP2, while always containing the BIR domains (1, 2, 4). Indeed, Hu and colleagues demonstrated that cIAP2-MALT1 is able to bind BCL10 but not able to ubiquitylate it (Figure 3B) (22). Interestingly, BCL10 and cIAP2-MALT1 synergistically activated NF-B, and, at least in HeLa cells, the downregulation of endogenous BCL10 caused a decreased response to NF-B activation induced by expression of the cIAP2-MALT1 fusion protein. These data indicate that BCL10 expression might be needed for cIAP2-MALT1 function. As a whole, the Hu et al. study (22) shows that cIAP2 as well as MALT1 and BCL10 is involved in physiologic antigen receptor stimulation. The EMZL-specific cIAP2-MALT1 fusion gene would behave both as a tumor suppressor gene, since it is no longer able to downregulate BCL10 expression, and also as an oncogene, since the fusion protein induces strong NF-B activation (22). These data are in perfect accordance with the high expression levels of BCL10 observed in clinical EMZL cases bearing the t(11;18)(q21;q21) translocation (9). The BCL10 protein is expressed only in the cytoplasm in non-neoplastic germinal center and marginal zone B cells as well as in EMZLs with neither t(1;14)(p22;q32) nor t(11;18)(q21;q21) translocations (9). Conversely, nuclear expression is present both in the t(1;14)-positive and in the t(11;18)-positive EMZLs, suggesting that nuclear localization of BCL10 can occur as the result of 2 apparently independent cytogenetic events. While the significance of this change in cellular localization is not yet known, a possible partial explanation has recently been presented by Nakagawa et al. (23), albeit in partial disagreement with the data reported here by Hu et al. (22). Because of the presence of 2 nuclear export signal (NES) domains (Figure 1A), MALT1 would export BCL10 from the nucleus to the cytoplasm (23). In the presence of the t(1;14)(p22;q32) chromosomal translocation, the overexpression of BCL10 would lead to increased BCL10 nuclear compartmentalization (13). In the presence of the t(14;18)(q32;q21) translocation, with increased WT MALT1 expression, the localization of BCL10 would remain cytoplasmic, as observed in normal B cells (23, 24). In the presence of the t(11;18)(q21;q21) translocation, the effect on BCL10 cellular localization is still unclear. While in the study reported by Nakagawa et al. (23) the cIAP2-MALT1 fusion protein has lost the ability to bind BCL10, possibly explaining the accumulation of BCL10 in the nucleus, according to Hu and colleagues (22) this protein would retain its ability to bind BCL10. Since the constructs used in the 2 studies are relatively comparable, differences in the cell systems in which the experiments have been carried out could at least partially explain the differences in the observed results. However, further studies of the significance of nuclear BCL10 expression are mandatory.
Outlook
Another field where additional studies will be welcome is the role played by antigens and by the concomitant chronic phlogistic status in the pathogenesis of EMZL. Indeed, EMZLs are considered, at least partially, to be antigen dependent. In addition to epidemiological and therapeutic evidence, another basis for this belief was the demonstration that EMZL cells would often recognize self-antigens (25, 26). However, a very recent publication by Lenze et al. (27) reported new data disproving a direct role for the Igs expressed by EMZLs in sustaining lymphoma growth. The fact that only 1 out of 7 lymphomas expressed specific Igs (actually, directed against a plasma cell protein) makes the direct influence of antigen or self-antigen much less important in sustaining the growth of lymphoma cells. Indeed, in gastric MALT lymphoma, H. pylori–specific T cells are necessary to sustain the in vitro growth of lymphoma cells (28). Considering also the extreme importance of non-neoplastic cells in the pathogenesis and the definition of prognosis of follicular lymphomas (another indolent B cell lymphoma) (29, 30), further investigation of nonlymphoma cells in EMZL appears worthwhile.
The 4 recurrent chromosomal translocations demonstrate a site-specificity in terms of their incidence (10). Indeed, another open question that remains unaddressed is the possible effect of infectious agents in inducing individual translocations and how lymphoma risk depends on the microenvironmental effects of the interaction between genetic factors of the microorganisms (11) and the genetic background of the host (31). Nevertheless, if bacterial and host factors have a relevant impact on the initiation of the lymphomagenesis process, the role of the genetic damage eventually acquired by the tumor lymphocytes is important as well. In this context, the data provided by Hu et al. (22) shed light on the molecular consequences of the most common translocation in MALT lymphoma, showing that the t(11;18)(q21;q21) fusion product (cIAP2-MALT1) may concomitantly contribute to the lymphomagenesis, acting either as a tumor suppressor gene (loss of function) or as an oncogene (gain of function). Indeed, as a result of the cIAP2-MALT1 fusion, cIAP2’s capacity for ubiquitylation and degradation of BCL10 is lost, and NF-B appears to be synergistically activated by BCL10 (which is no longer degraded) and cIAP2-MALT1. The combination of both properties of cIAP2-MALT1 appears crucial in the strong NF-B activation observed in lymphocytes harboring the t(11;18)(q21;q21) translocation, but the relative contribution to the oncogenic potential of these 2 properties of cIAP2-MALT1 remains to be determined.
Footnotes
Nonstandard abbreviations used: BIR, baculoviral IAP repeat; CARD, caspase recruitment domain; cIAP2, cellular inhibitor of apoptosis protein 2; DD, death domain; EMZL, extranodal marginal zone B cell lymphoma; IAP, inhibitor of apoptosis protein; IB, inhibitory B; IKK, IB kinase; MALT, mucosa-associated lymphoid tissue; NES, nuclear export signal.
Conflict of interest: The authors have declared that no conflict of interest exists.
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