ZHU Liª²Hua1, LI Shuª²Ying1, ZHOU Hongª²Xia2, XI Jinª²Kun3, ZHANG Guangª²Ling1, ZHOU Tianª²Ji4

    1Department of Pathobiology, Faculty of Bioscience, 2Department of Anatomy, Faculty of Basic Medicine, 3Central Laboratory, North China Coal Medical College, Tangshan 063000, China, 4Department of Pathobiology, School of Medical Technology, Jiangsu University, Zhenjiang 212000, China

    ¡¾Abstract¡¿ AIM£ºTo observe the effect of bclª²2 gene transfection on the proliferation of human gastric epithelial cell GESª²1. METHODS£ºThe eukaryotic vector carrying the full length of bclª²2 gene and the neomycin resistance gene (pcDNA3/bclª²2) and the one only carrying the neomycin resistance gene (pcDNA3) were amplified and purified. The plasmid of pcDNA3/bclª²2 was used to transfect GESª²1 cells by using the lipofectamine, and the empty vector pcDNA3 to transfect GESª²1 cells as controls. And then, the cells were cultured in 1640 culture medium containing an appropriate concentration of G418 for selection. The method of HRP label immunohistochemical staining was used to identify the cells expressing bclª²2 gene positively. The morphologic changes of GESª²1 cells were observed under an optical microscope by HE staining. The cell proliferation affected by the transfection of bclª²2 was measured by cell counting and MTT assay. RESULTS: bclª²2 gene and the vector pcDNA3 were transfected separately into GESª²1 cells by lipofectamine. After G418 selection, the cells were transfected steadily. Compared with controls, HRP label immunohistochemical staining showed that bclª²2 gene transfected cells expressed Bclª²2 protein much more obviously. No morphologic changes were observed by HE staining. The growth curve was drawn by the method of cell counting ......