ZHANG Xiaoª²Zhan1, NAN Keª²Jun2, LI Chunª²Li2£¬CHEN Ling2

    1Department of Oncology, Fifth Affiliated Hospital, Zunyi Medical College, Zhuhai 519100, China, 2Department of Oncology, First Affiliated Hospital, Medical College, Xi¬ðan Jiaotong University, Xi¬ðan 710061, China

    ¡¾Abstract¡¿ AIM: To study the expression of p34cdc2 in hepatocellular carcinoma (HCC) and the relationship between the expression of p34cdc2 and proliferative index (PI)£¬ apoptotic index (AI) of HCC. METHODS: Fortyª²seven cases of HCC with 42 specimens of neighboring nonª²cancerous tissues and 10 healthy hepatocellular tissues were selected for the study. These samples were detected by Sª²P immunohistochemical and TUNEL methods. All data were processed by SPSS 10.0 version in computer. RESULTS: The expression of p34cdc2 was localized in cytoplasm and nuclear of HCC tissues, and neighboring nonª²cancerous tissues, showing brownish yellow or yellow. There was p34cdc2 overexpression in HCC, compared to normal liver tissues (P<0.01). PI of HCC and neighboring nonª²cancerous tissue was (30.34¡À4.46)% and (27.88¡À5.89)% respectively. AI of HCC and neighboring nonª²cancerous tissue was (8.62¡À2.28)% and (7.38¡À2.61)% respectively. AI and PI in HCC were higher than those in neighboring nonª²cancerous tissue (P<0.05). PI was significantly higher in p34cdc2 protein positive tissues [(49.26¡À4.81)%] than in p34cdc2 protein negative tissues [(23.07¡À4.15)%] (P<0.05), but p34cdc2 protein expression was not significantly related to AI (P>0.05). CONCLUSION: There is an overexpression of p34cdc2 in HCC. AI and PI in HCC are both higher than those in neighboring nonª²cancerous tissue. Although it is not a prerequisite for HCC cell apoptosis, p34cdc2 expression is positively associated with PCNA expression in HCC, and can together with PCNA serve as a sensitive parameter for detecting HCC cell proliferation. ......