[摘要]目的 观察人胎脑组织有无神经巢蛋白(Nestin)阳性细胞存在。方法 收集胎龄为3个月左右的自愿终止妊娠引产的正常胚胎3例。应用苏木精-伊红染色和免疫组织化学方法观察人胎脑室管膜下区、海马、纹状体、丘脑、嗅球、皮质、脑干及小脑的Nestin阳性细胞。结果分别在人胎脑室管膜下区、海马、纹状体、丘脑、嗅球、皮质部位发现数量不等的Nestin阳性表达细胞，而在脑干、小脑未发现Nestin阳性细胞。结论 人胎脑室管膜下区、海马、纹状体、丘脑、嗅球、皮质多个部位存在数量不等的神经干细胞。

    [关键词] 干细胞;神经巢蛋白;胚胎;神经组织;免疫组织化学

    POSITIVE EXPRESSION OF NESTIN IN HUMAN FETAL BRAIN AND ITS SIGNIFICANCE

    LIU YU-XIA， FAN XING-LONG， JIANG CHEN， et al

    (Department of Neurology， Qingdao Municipal Hospital， Qingdao 266011， China)

    [ABSTRACT]ObjectiveTo observe the presence or absence of nestin cells in different parts of human fetal brain. Me- thodsThree normal embryos of three-month old were collected from voluntary induced labor. The cells from hippocampus， stria-tum， ependyma， thalamus， olfactory bulb， cortex， brain stem and cerebellum of the brain were studied using HE staining and im-munocytochemistry. ResultsDifferent amounts of positive nestin cells were detected in the hippocampus， striatum， subependy-mal region， thalamus， olfactory bulb ......