摘要:目的 探讨聚合酶链反应(PCR)技术诊断单纯疱疹(单疱)病毒性角膜炎的实用价值，为临床诊断提供依据和方法。 方法 应用PCR技术对60例按临床诊断标准诊断为单疱病毒性角膜炎患者的患眼泪液及角膜上皮进行病毒DNA检测。 结果 PCR检测60例患者标本结果，阳性46例占76.7%，阴性14例占23.3%。 结论 对单疱病毒性角膜炎诊断不能只根据其临床特征来诊断;PCR可为临床提供实验诊断依据。

    关键词:单疱病毒;角膜炎;泪液和角膜上皮;聚合酶链反应(PCR)

    Diagnosis of herpes simplex keratitis by using PCR.

    ZHUQi-xin，YU Wo-lian.

    (Zhaoqing Municipal Second People's Hospital，Zhaoqing526060，Guangdong，P.R.China)

    Abstract:Objective To detect herpes simplex virus(HSV)in tear and corneal epithelium of patients with herpes simlex ker-atitis(HSK). Methods Tear and corneal epithelium of60cases clinically diagnosed as HSKwere tested by polymerase chain re-action(including24cases of punctate keratopathy ......