[摘 要] 目的: 研究低氧预适应诱导神经胶质细胞瘤细胞(C6 Glioma Cells，简称C6细胞)对氧葡萄糖剥夺(OGD) 的耐受及其机制。 方法: C6细胞通过低氧(1%)预处理，在经受OGD打击后，观测其对细胞活力的影响。利用West- ern blot分析低氧预适应对C6细胞磷酸化-ERK(p-ERK)、缺氧诱导因子-1α(HIF-1α)和红细胞生成素(EPO)蛋白的 影响;通过PD98059和LY294002的使用，分析低氧诱导C6细胞预适应现象与MEK/ERK通路的关系。 结果: 低氧能 诱导细胞预适应的发生，而且对于24 h的OGD，最有效的低氧预适应时间为16 h;低氧能诱导细胞p-ERK信号蛋白 的上调，激活HIF-1α和增加EPO的表达，且能被PD98059所抑制。 结论: 低氧预适应能增强C6细胞对24 hOGD的 耐受，产生预保护作用，其中以低氧预适应16 h效率最高;MEK/ERK信号转导途径参与了低氧预适应作用;低氧预适 应可通过激活HIF-1α和增加EPO表达水平来实现。

    [关键词] 低氧; 预适应; 氧葡萄糖剥夺; MEK/ERK; 低氧诱导因子-1α

    Hypoxia preconditioning induces tolerance against oxygen glucose deprivation in C6 glioma cells

    HE Wei， ZHU Li， JIN Shu-yi

    (1.School of Nursing ;2.Institute of Nautical Medicine， Nantong University， Nantong Jiangsu 226001，China) [Abstract] Objective: To investigate the tolerance against OGD induced by hypoxia preconditioning and its mechanism. Methods: The C6 cellular viability changes were observed after the pretreatment of hypoxia(1% O2) and the attack of OGD. Using Western blot， we analyzed the influence on the expression of p- ERK， HIF-1α and EPO proteins in C6 cells pretreated by hypoxia. The relationship between the tolerance against OGD induced by hypoxia preconditioning and the MEK/ERK pathway was elucidated in the pres- ence of PD98059 and LY294002. Results: Hypoxia preconditioning can induce protection against OGD. The most effective duration of hypoxia preconditioning against 24 h-OGD is 16 hours. Hypoxia precondition-ing could up-regulate p-ERK signal protein ......