The histone deacetylase inhibitor trichostatin A alters the pattern of
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《核酸研究医学期刊》
The authors would like to apologize for errors in the legends to Figures 6 and 8. The two correct legends are given below.
Figure 6. TSA treatment alters the pattern of replication origin activity in HeLa cells. Asynchronously growing HeLa cells were treated for 4, 8 or 24 h with 100 ng/ml TSA and loaded on alkaline gels for isolation of 1–2 kb nascent DNA. Purified nascent DNA was quantitated by OliGreen fluorescence and quantitated by real-time PCR. The data presented are the averages (and SD values) of triplicate analysis on at least three independent preparations of nascent DNA.
Figure 8. TSA alters nascent strand abundance at uncharacterized sites. Nascent DNA isolated from untreated cells or cells treated with 100 ng/ml TSA for 4, 8, 24 or 48 h was amplified by PCR with 10mer primer 1 (A), 10mer primer 2 (B) or a mixture of primers 1 and 2 (C) using equal starting amounts of DNA. Products were purified, run on 2% agarose gels and stained with EtBr (inverse image). Samples from independent PCRs are shown to confirm the reproducibility of the assay.(Michael G. Kemp, Maloy Ghosh, Guoqi Liu )
Figure 6. TSA treatment alters the pattern of replication origin activity in HeLa cells. Asynchronously growing HeLa cells were treated for 4, 8 or 24 h with 100 ng/ml TSA and loaded on alkaline gels for isolation of 1–2 kb nascent DNA. Purified nascent DNA was quantitated by OliGreen fluorescence and quantitated by real-time PCR. The data presented are the averages (and SD values) of triplicate analysis on at least three independent preparations of nascent DNA.
Figure 8. TSA alters nascent strand abundance at uncharacterized sites. Nascent DNA isolated from untreated cells or cells treated with 100 ng/ml TSA for 4, 8, 24 or 48 h was amplified by PCR with 10mer primer 1 (A), 10mer primer 2 (B) or a mixture of primers 1 and 2 (C) using equal starting amounts of DNA. Products were purified, run on 2% agarose gels and stained with EtBr (inverse image). Samples from independent PCRs are shown to confirm the reproducibility of the assay.(Michael G. Kemp, Maloy Ghosh, Guoqi Liu )