Oxidation makes a germline
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《细胞学杂志》
Hekimi/AAAS
Food and sex go hand in hand, according to Yukimasa Shibata, Siegfried Hekimi, and colleagues (McGill University, Montreal, Canada). They find that worms that have less oxidation of certain lipoprotein particles—possibly an indicator of a slowed metabolism—have slowed development of their germline. Only when food is abundant and metabolism active would the germline get the stimulus to develop to maturity.
The proteins in question are vitellogenins: analogues of vertebrate apoB, a component of low-density lipoprotein (LDL). In the clk-1 worm mutant, an increase in the levels of an antioxidant results in less oxidation of lipoprotein. The result is a slowing in germline development.
The slowed development is reversed by blocking the production of lipoproteins, or by reestablishing a more normal level of oxidation by reactive oxygen species (ROS). "The degree of oxidation is a measure of general metabolism," says Hekimi. "The germline may want to know that the worm is running fast. It could be sensing the general quality of metabolism." ROS effects on signaling have been seen before in vitro, but the new results are the most dramatic to be detected in vivo.
The effects of lipoproteins on germline development go through a receptor-associated kinase called ARK-1. Although ARK-1 is known to work downstream of an EGF-like receptor, it is not clear whether this or another type of receptor is a mediator for the lipoprotein signal, or which lipoprotein species (oxidized or nonoxidized) is doing the signaling.
Reference:
Shibata, Y., et al. 2003. Science. 302:1779–1782.(Oxidized LDL may signal that metabolism )
Food and sex go hand in hand, according to Yukimasa Shibata, Siegfried Hekimi, and colleagues (McGill University, Montreal, Canada). They find that worms that have less oxidation of certain lipoprotein particles—possibly an indicator of a slowed metabolism—have slowed development of their germline. Only when food is abundant and metabolism active would the germline get the stimulus to develop to maturity.
The proteins in question are vitellogenins: analogues of vertebrate apoB, a component of low-density lipoprotein (LDL). In the clk-1 worm mutant, an increase in the levels of an antioxidant results in less oxidation of lipoprotein. The result is a slowing in germline development.
The slowed development is reversed by blocking the production of lipoproteins, or by reestablishing a more normal level of oxidation by reactive oxygen species (ROS). "The degree of oxidation is a measure of general metabolism," says Hekimi. "The germline may want to know that the worm is running fast. It could be sensing the general quality of metabolism." ROS effects on signaling have been seen before in vitro, but the new results are the most dramatic to be detected in vivo.
The effects of lipoproteins on germline development go through a receptor-associated kinase called ARK-1. Although ARK-1 is known to work downstream of an EGF-like receptor, it is not clear whether this or another type of receptor is a mediator for the lipoprotein signal, or which lipoprotein species (oxidized or nonoxidized) is doing the signaling.
Reference:
Shibata, Y., et al. 2003. Science. 302:1779–1782.(Oxidized LDL may signal that metabolism )