刺五加；_高效液相色谱；_指纹图谱____

刺五加药材指纹图谱的分析方法研究

http://www.100md.com 《时珍国医国药》 2006年第9期

刺五加；,高效液相色谱；,指纹图谱,,刺五加；,高效液相色谱；,指纹图谱,1仪器与试药,2方法,3结果,4讨论,参考文献：

     摘要：目的采用高效液相色谱(HPLC)法建立了刺五加药材指纹图谱。方法色谱柱: Zorbax SBC18柱(250 mm×4.6 mm，5 μm)，流动相∶乙腈水(梯度洗脱)，检测波长：203 nm，柱温：40℃，流速：1.0 ml/min，分析时间：90 min。结果共标示出刺五加药材指纹图谱中24个共有峰。利用指纹图谱相似度测试软件，以夹角余旋为测度，测得10批刺五加药材指纹图谱相似度结果均在0.90～1.00之间。结论本实验所建立的刺五加药材指纹图谱分析方法准确、重复性好，为有效控制刺五加药材的质量提供了依据。

    关键词：刺五加；高效液相色谱；指纹图谱

    Studies on the Analysis Methods of Chromatographic Fingerprints of Acanthopanax senticosus Harms

    GAO Yang， ZHAO Yunli， BI Kaishun， YU Zhiguo*

    (School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China)

    Abstract：ObjectiveTo establish chromatographic fingerprint of Acanthopanax senticosus Harms by HPLC. MethodsSeparation was performed on an Agilent ZORBAX SBC18 (4.6 mm×250 mm, 5 μm) analytical column with mobile phase consisting of acetonitrile and water with gradient elute at the flow rate of 1.0 ml/min and the column temperature 40℃. The UV wavelength used for detection was set at 203 nm and the analysis time was 90 min.Results24 copeaks on the HPLC fingerprints of Acanthopanax senticosus Harms were indicated. The similarities were determined by cosine. The results of similarity analysis were 0.90～1.00. ConclusionThe method can be used for the quality control of Acanthopanax senticosus Harms. ......

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