枳实药材中总黄酮提取工艺的研究
枳实；,总黄酮；,提取工艺；,正交实验,,枳实；,总黄酮；,提取工艺；,正交实验,1仪器与试药,2方法,3枳实总黄酮提取工艺的研究,4讨论,参考文献：
摘要：目的优化枳实总黄酮最佳提取工艺。方法以柚皮苷为对照，用紫外分光光度法在283 nm处测定总黄酮含量，以总黄酮含量为指标，应用正交实验，优选枳实总黄酮最佳提取工艺，并对总黄酮水洗除杂的工艺进行了优化。结果优选出的最佳提取工艺是：60%乙醇，8倍量，回流提取2次，每次1.5 h。水洗除杂工艺为：9倍量水静置24 h，共2次。结论 提取的总黄酮含量达到了70%，工艺简便、可行，生产成本低，可供工业化生产参考。关键词：枳实； 总黄酮； 提取工艺； 正交实验
Optimization of the Extracting Method of Total Flavonoids from Frutus aurantii immaturus
LIU Wenjuan, GENG Qiuju, CAI Rong, YAO Daolu
(Hebei Yiling Academy of Medicine, Shijiazhuang, 050035, China)
Abstract：ObjectiveTo optimize the extraction procedure of the total flavonoids from Frutus aurantii immaturus. MethodsExtraction procedure was screened by orthogonal test according to the content of the total flavonoids which was measured through UV spectrophotometry at 283 nm using naringin as control, and the procedure of removing impurity was screened by water.ResultsThe optimized extraction procedure was as follows: 60% alcohol, 8 times volume of the weight of the dried material, refluxing 1.5 h for 2 times. The procedure of removing impurity in water was as follows: 9 times volume of the weight of the dried material, placed 24 h for 2 times.ConclusionThe content of the total flavonoids is above 70%. The procedure is simple and feasible. The production cost is low. The procedure can provide a reference to the production. ......
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