添加益生元肠内营养对重症急性胰腺炎肠屏障损害和内毒素易位的作用
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王 慧, 钟 燕, 蔡东联, 耿珊珊, 韩 婷, 陈凌云
重症急性胰腺炎;肠内营养;益生元;肠屏障损害;内毒素易位,王慧,钟燕,蔡东联,耿珊珊,韩婷,陈凌云,王慧,通讯作者:,Enteralnutritionwithprebioticsonintestinalbarrierdy
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王慧, 中国人民解放军第一五三医院营养科 河南省郑州市 450052
钟燕, 上海交通大学医学院营养系 上海市 200092
蔡东联, 耿珊珊, 韩婷, 陈凌云, 第二军医大学附属长海医院临床营养中心 上海市 200433
王慧, 1998年河南中医学院学士, 主要从事临床营养研究.
上海市科学技术委员会科研计划项目, No. 034119829
通讯作者: 钟燕, 200092, 上海市控江路1665号, 上海交通大学医学院营养系. zhongyan73@263.net
电话: 021-65790000-3315
收稿日期: 2006-12-20 接受日期: 2007-01-10
Enteral nutrition with prebiotics on intestinal barrier dysfunction and endotoxin translocation in severe acute pancreatitis
Hui Wang, Yan Zhong, Dong-Lian Cai, Shan-Shan Geng, Ting Han, Ling-Yun Chen
Hui Wang,Department of Nutrition, the 153 Hospital of Chinese PLA, Zhengzhou 450052, He’nan Province, China
Yan Zhong,Department of Nutrition, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China
Dong-Lian Cai, Shan-Shan Geng, Ting Han, Ling-Yun Chen,Center of Clinical Nutrition, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai 200433, China
Supported bythe Science Research Project of Shanghai Science and Technology Committee, No. 034119829
Correspondence to:Dr. Yan Zhong, Department of Nutrition, School of Medicine, Shanghai Jiao Tong University, 1665 Kongjiang Road, Shanghai 200092, China. zhongyan73@263.net
Received:2006-12-20 Accepted:2007-01-10
Abstract
AIM: To explore the effect of micro-ecological enteral nutrition (EN) with prebiotics on the intestinal barrier dysfunction and endotoxin translocation in rats with severe acute pancreatitis (SAP).
METHODS: SAP specific enteral nutrition formula (EN-S) was prepared based on the nutritional and metabolic characteristic of SAP, and the micro-ecological EN formula was prepared with EN-S plus prebiotics (PRE-EN). Sixty-four Spraugue Dawley rats were divided randomly and averagely into 8 groups: sham operation control 4-day group, sham operation control 7-day group, SAP with EN-S 4-day group, SAP with EN-S 7-day group, SAP with PRE-EN 4-day group, SAP with PRE-EN 7-day group, SAP with PN 4-day group, and SAP with PN 7-day group, named as group A, B, C, D, E, F, G and H, respectively. The rat model of SAP was established by injection of 38 g/L sodium taurocholate (1 mL) beneath the pancreatic capsule. The nutritional therapy began after SAP model was established. The rats were killed on day 4 and 7, respectively. The serum levels of endotoxin and diamine oxidase (DAO) were detected. The apoptosis of intestinal mucosal epithelial cells were analyzed with terminal deoxynucleotidyl-transferase mediated dUTP-biotin nick and labeling (TUNEL) method, and the apoptosis index (AI) was calculated.
RESULTS: At each time point, the levels of plasma endotoxin and DAO, and the value of AI were significantly higher in group C (158.7 ± 23.9 EU/L, 5.17 ± 0.62 kU/L, 50.5% ± 9.7%), D (140.5 ± 20.9 EU/L, 4.09 ± 0.49 kU/L, 41.6% ± 7.8%), E (110.4 ± 14.4 EU/L, 3 ......
王慧, 中国人民解放军第一五三医院营养科 河南省郑州市 450052
钟燕, 上海交通大学医学院营养系 上海市 200092
蔡东联, 耿珊珊, 韩婷, 陈凌云, 第二军医大学附属长海医院临床营养中心 上海市 200433
王慧, 1998年河南中医学院学士, 主要从事临床营养研究.
上海市科学技术委员会科研计划项目, No. 034119829
通讯作者: 钟燕, 200092, 上海市控江路1665号, 上海交通大学医学院营养系. zhongyan73@263.net
电话: 021-65790000-3315
收稿日期: 2006-12-20 接受日期: 2007-01-10
Enteral nutrition with prebiotics on intestinal barrier dysfunction and endotoxin translocation in severe acute pancreatitis
Hui Wang, Yan Zhong, Dong-Lian Cai, Shan-Shan Geng, Ting Han, Ling-Yun Chen
Hui Wang,Department of Nutrition, the 153 Hospital of Chinese PLA, Zhengzhou 450052, He’nan Province, China
Yan Zhong,Department of Nutrition, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China
Dong-Lian Cai, Shan-Shan Geng, Ting Han, Ling-Yun Chen,Center of Clinical Nutrition, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai 200433, China
Supported bythe Science Research Project of Shanghai Science and Technology Committee, No. 034119829
Correspondence to:Dr. Yan Zhong, Department of Nutrition, School of Medicine, Shanghai Jiao Tong University, 1665 Kongjiang Road, Shanghai 200092, China. zhongyan73@263.net
Received:2006-12-20 Accepted:2007-01-10
Abstract
AIM: To explore the effect of micro-ecological enteral nutrition (EN) with prebiotics on the intestinal barrier dysfunction and endotoxin translocation in rats with severe acute pancreatitis (SAP).
METHODS: SAP specific enteral nutrition formula (EN-S) was prepared based on the nutritional and metabolic characteristic of SAP, and the micro-ecological EN formula was prepared with EN-S plus prebiotics (PRE-EN). Sixty-four Spraugue Dawley rats were divided randomly and averagely into 8 groups: sham operation control 4-day group, sham operation control 7-day group, SAP with EN-S 4-day group, SAP with EN-S 7-day group, SAP with PRE-EN 4-day group, SAP with PRE-EN 7-day group, SAP with PN 4-day group, and SAP with PN 7-day group, named as group A, B, C, D, E, F, G and H, respectively. The rat model of SAP was established by injection of 38 g/L sodium taurocholate (1 mL) beneath the pancreatic capsule. The nutritional therapy began after SAP model was established. The rats were killed on day 4 and 7, respectively. The serum levels of endotoxin and diamine oxidase (DAO) were detected. The apoptosis of intestinal mucosal epithelial cells were analyzed with terminal deoxynucleotidyl-transferase mediated dUTP-biotin nick and labeling (TUNEL) method, and the apoptosis index (AI) was calculated.
RESULTS: At each time point, the levels of plasma endotoxin and DAO, and the value of AI were significantly higher in group C (158.7 ± 23.9 EU/L, 5.17 ± 0.62 kU/L, 50.5% ± 9.7%), D (140.5 ± 20.9 EU/L, 4.09 ± 0.49 kU/L, 41.6% ± 7.8%), E (110.4 ± 14.4 EU/L, 3 ......
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