Timeª²resolved fluoroimmunoassay of high sensitive Cª²reactive protein

    HANG Jianª²Feng, WU Yingª²Song, XU Weiª²Wen, ZHOU Zhiª²Cong, LI Ming

    School of Biotechnology, Southern Medical University, Guangzhou 510515, China, Guangzhou Darui Antibody CO., LTD, Guangzhou 510663, China

    ¡¾Abstract¡¿ AIM: To establish twoª²site timeª²resolved fluoroimmunoassay (TRFIA) of high sensitive Cª²reactive protein (CRP) in serum based on direct sandwich technique. METHODS: The sandwich technique was used to detect serum CRP level (One mAb was immobilized on the surface of microtiter plate wells, and the other mAb was labelled with Eu3+). RESULTS: The linear measurement range of CRPª²TRFIA was 1-1000 mg/L. The sensitivity was 0.06 mg/L. The intraª² and interª²assay coefficients of variation were 4.0%-7.2% and 6.7%-10.8%, respectively. There was no cross reactivity with human IgG and albumin. The correlation coefficient of 109 blood samples detection results between CRPª²TRFIA and commercially available CRPª²ELISA kit(IBL) was 0.916. CONCLUSION: CRPª²TRFIA is a new immunoassay which has several advantages including high sensitivity, specificity, and wide working range. It is suitable for routine clinical use instead of hsª²CRP kit produced by IBL company. ......