恶性浆膜腔积液脱落细胞中P21 ras蛋白表达状况及其意义
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宋荣峰, 张焜和, 靳文剑, 陈 江, 祝金泉, 吕农华
P21ras蛋白;浆膜腔积液;免疫细胞化学染色,恶性浆膜腔积液脱落细胞中P21ras蛋白表达状况及其意义,宋荣峰,靳文剑,张焜和,陈江,祝金泉,吕农华,通讯作者:,电话:,收稿日期:,接受日期:,ExpressionofP21rasproteininmalignantserouscav
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宋荣峰, 靳文剑, 江西省肿瘤医院内科 江西省南昌市 330029
张焜和, 陈江, 祝金泉, 吕农华, 南昌大学第一附属医院消化病研究所 江西省南昌市 330006江西省科技厅科技攻关项目, No. F980508
通讯作者: 张焜和, 330006, 江西省南昌市永外正街, 南昌大学第一附属医院消化研究所. yfyzkh@sina.com
电话: 0791-8692507
收稿日期: 2006-11-15 接受日期: 2006-12-18
Expression of P21 ras protein in malignant serous cavity effusions and its significance
Rong-Feng Song, Kun-He Zhang, Wen-Jian Jin, Jiang Chen, Jin-Quan Zhu, Nong-Hua Lv
Rong-Feng Song, Wen-Jian Jin,Department of Internal Medicine, Jiangxi Tumor Hospital, Nanchang 330029, Jiangxi Province, China
Kun-He Zhang, Jiang Chen, Jin-Quan Zhu, Nong-Hua Lv,Institute of Digestive Diseases, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
Supported by the Key Science and Technology Development Program of Jiangxi Province No. F980508
Correspondence to:Kun-He Zhang, Institute of Digestive Diseases, the First Affiliated Hospital of Nanchang University, Yongwai Street, Nanchang 330006, Jiangxi Province, China. yfyzkh@sina.com
Received:2006-11-15 Accepted:2006-12-18
AbstractAIM:To investigate the expression of P21 ras protein in the exfoliative cells from malignant serous cavity effusions and its diagnostic value.
METHODS: Exfoliative cells in fresh specimens of serous cavity effusions were collected after centrifugation, and cell smears were prepared for cytological diagnosis, by which the specimens were divided into benign or malignant groups. The remained exfoliative cells were processed with a “standardized” procedure: removing red cells, fixing in paraformaldehyde, adjusting the cell concentration, and finally, cell smears were prepared and P21 ras immunocytochemistry were performed (SP method).
RESULTS: A total of 108 effusion specimens were collected, including 53 malignant and 55 benign one. In the immunocytochemical staining of P21 ras, 39 of 53 malignant effusions were positive (73.6%), and most of them were strongly positive. The positive results were observed in 12 of 55 (21.8%) benign effusions, most of which were weakly positive. The positive rates (χ2 = 29.02, P < 0.001) and expression intensity (Uc = 6.786, P < 0.001) of P21 ras were significantly different between malignant and benign effusions. The sensitivity, specificity and diagnostic accordance rate of P21 ras immunocytochemistry in the diagnosis of malignant effusions were 73.6%, 78.2% and 75.9%, respectively.
CONCLUSION: P21 ras immunocytochemical staining of exfoliative cells is positive in most malignant effusions, but negative in most benign ones, implicating that it is valuable for the diagnosis of malignant effusions.
Key Words: P21 ras protein; Serous cavity effusion; Immunocytochemistry
Song RF, Zhang KH, Jin WJ, Chen J, Zhu JQ, Lv NH. Expression of P21 ras oncoprotein in malignant serous cavity effusions and its significance ......
宋荣峰, 靳文剑, 江西省肿瘤医院内科 江西省南昌市 330029
张焜和, 陈江, 祝金泉, 吕农华, 南昌大学第一附属医院消化病研究所 江西省南昌市 330006江西省科技厅科技攻关项目, No. F980508
通讯作者: 张焜和, 330006, 江西省南昌市永外正街, 南昌大学第一附属医院消化研究所. yfyzkh@sina.com
电话: 0791-8692507
收稿日期: 2006-11-15 接受日期: 2006-12-18
Expression of P21 ras protein in malignant serous cavity effusions and its significance
Rong-Feng Song, Kun-He Zhang, Wen-Jian Jin, Jiang Chen, Jin-Quan Zhu, Nong-Hua Lv
Rong-Feng Song, Wen-Jian Jin,Department of Internal Medicine, Jiangxi Tumor Hospital, Nanchang 330029, Jiangxi Province, China
Kun-He Zhang, Jiang Chen, Jin-Quan Zhu, Nong-Hua Lv,Institute of Digestive Diseases, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
Supported by the Key Science and Technology Development Program of Jiangxi Province No. F980508
Correspondence to:Kun-He Zhang, Institute of Digestive Diseases, the First Affiliated Hospital of Nanchang University, Yongwai Street, Nanchang 330006, Jiangxi Province, China. yfyzkh@sina.com
Received:2006-11-15 Accepted:2006-12-18
AbstractAIM:To investigate the expression of P21 ras protein in the exfoliative cells from malignant serous cavity effusions and its diagnostic value.
METHODS: Exfoliative cells in fresh specimens of serous cavity effusions were collected after centrifugation, and cell smears were prepared for cytological diagnosis, by which the specimens were divided into benign or malignant groups. The remained exfoliative cells were processed with a “standardized” procedure: removing red cells, fixing in paraformaldehyde, adjusting the cell concentration, and finally, cell smears were prepared and P21 ras immunocytochemistry were performed (SP method).
RESULTS: A total of 108 effusion specimens were collected, including 53 malignant and 55 benign one. In the immunocytochemical staining of P21 ras, 39 of 53 malignant effusions were positive (73.6%), and most of them were strongly positive. The positive results were observed in 12 of 55 (21.8%) benign effusions, most of which were weakly positive. The positive rates (χ2 = 29.02, P < 0.001) and expression intensity (Uc = 6.786, P < 0.001) of P21 ras were significantly different between malignant and benign effusions. The sensitivity, specificity and diagnostic accordance rate of P21 ras immunocytochemistry in the diagnosis of malignant effusions were 73.6%, 78.2% and 75.9%, respectively.
CONCLUSION: P21 ras immunocytochemical staining of exfoliative cells is positive in most malignant effusions, but negative in most benign ones, implicating that it is valuable for the diagnosis of malignant effusions.
Key Words: P21 ras protein; Serous cavity effusion; Immunocytochemistry
Song RF, Zhang KH, Jin WJ, Chen J, Zhu JQ, Lv NH. Expression of P21 ras oncoprotein in malignant serous cavity effusions and its significance ......
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