葡萄球菌；鉴定方法；凝固酶；凝集因子____

三种鉴定葡萄球菌凝固酶方法的评价

http://www.100md.com 《实用医技杂志》 2007年第2期

葡萄球菌；鉴定方法；凝固酶；凝集因子,,],葡萄球菌；鉴定方法；凝固酶；凝集因子,1材料和方法,2结果,3讨论,参考文献：

     [摘要] 目的：评价3种鉴定葡萄球菌凝固酶方法的敏感性和特异性。方法：临床分离的110株葡萄球菌，经革兰染色、触酶试验、ARIS2X鉴定后，用试管法、玻片法、商品乳胶血凝法(slidex staphkit)和凝固酶基因法，鉴定葡萄球菌和评价凝固酶试验方法。4种方法结果不一致的菌株进行重复试验。用凝固酶基因法作为“金标准”，比较其他3种凝固酶方法的敏感性、特异性。结果：凝固酶基因法能够正确判定葡萄球菌凝固酶；试管法有1株菌鉴定假阴性；玻片法有8株菌假定假阳性，2株假阴性；乳胶血凝法有2株假阳性。试管法、玻片法、乳胶血凝法的敏感性分别为98.53%、97.30%和100%；特异性分别为100%、77.78%和94.44%。玻片法鉴定葡萄球菌凝固酶有9.01%的错误。结论：乳胶血凝法和试管法敏感性和特异性较高，是鉴定葡萄球菌凝固酶试验的好方法，玻片法的特异性较差，可能是由于人血浆中产生的葡萄球菌抗体所致。

    [关键词] 葡萄球菌；鉴定方法；凝固酶；凝集因子

    The Evaluation of 3 Kinds Methods Authenticating Staphylococci Coagulase

    ZHANG Hongsheng

    (The People Hospital of Anyang City, Anyang，He'nan 455000，China).

    Abstract： Objective Evaluates it's sensitivity and particularity 3 kinds of staphylococci coagulas methods .Methods The method is clinical disjunctive 110 stub staphylococcus, after Gram stainhucker modification, Catalase test, ARIS2X authentication ,using the rube method ,the slide agglutination test method ,the sidex staphkit method and coagulase gene method, to authenticate staphylococci and evaluate coagulase test method. The strain will be test again if the result of 4 kind method are inconsistent. As the Coagulasegene method is a "gold standard", evaluate it's sensitivity and particularity lf the 3 kinds of coagulase methods .Results The Coagrlasegene method can authenticate staphylococci coagulase correctly ;There are 1 strain fake negative result by the tube method; 8 strains fake positive result ,2strain fade negative result by agglutination test method;2 strains fake positive result .It's sensitivity is 98.53%、97.30%and 100%,specificity is 100%、77.78%and 94.44%correspond with the tube method ,the slide agglutination test method, the slidex staph kit method and coagulase gene method. There are 9.01% wrong result by using agglutination test method authenticating staphylococci. Conclusion It's good method to authenticate staphylococci coagulase test by the slidex staphkit method and the tube method with highly sensitivity and specificity .Otherwise ,the specificity of the slide agglutination test method is lower than the others ,perhaps it caused by staphylococci antibody in human plasm. ......

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