The effect of morin on activation, proliferation and cellª²cycle of murine T lymphocytes in vitro

    ZANG Ning, ZENG Yaoª²ying*, HUANG Xiuª²yan, WANG Tong, YE Xueª²yi, ZHOU Jianª²guo, WANG Huiª²ying

    Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China

    [Abstract] AIM: To discover the effects of morin on the activation, proliferation and cellª²cycle of murine T lymphocytes in vitro. METHODS: Murine lymph nodeª²derived T lymphocytes were separated and stimulated with concanavalin A (ConA) and different experimental groups were set by coª²cultured with morin of different final concentration. Flow cytometry (FCM) was used to detect the activation, proliferation [carboxylfluorescein diacetate, succinimide ester (CFDAª²SE) staining] and cellª²cycle [propidium iodide(PI) staining] of T cells. RESULTS: After 6 h time of culture in vitro, the rate of CD69+ T cells in control group was (2.97¡À0.12)%, while it was significant higher in ConA group[(72.52¡À0.66)% (P<0.01)]. Morin could downª²regulate this rate at final concentration being 25, 50 and 100 ¦Ìmol/L, with a peak at 100 ¦Ìmol/L morin[(48.95¡À0.81)% (P<0.01)]. CFDAª²SE staining showed that at 48 h and 72 h, the proliferation indexes (PI) of T cells in ConA group were (1.58¡À0.04) and (1.95¡À0.02), respectively. Morin could significantly decrease the PI value at all experimental concentration, with the peak effect at 100 ¦Ìmol/L morin, which the PI for 48 h was (1.02¡À0.02) and (1.03¡À0.01) for 72 h (P<0.01). FCM analysis of PI staining implied that the percentage of S phase cells in ConA group was (27.05¡À0.39)%, significantly higher than that in control group (5.10¡À0.07)%; and the 25 and 50 ¦Ìmol/L morin groups showed higher S phase cell rates. CONCLUSION: Morin can significantly inhibit ConA stimulated activation and proliferation of murine T lymphocytes, in which the S phase lagging may serve as one of the major mechanisms. ......