醛固酮对肾小球系膜细胞合成分泌纤维连接蛋白和TGFβ1表达的影响
醛固酮;,肾小球系膜细胞;,纤维连接蛋白;,转化生长因子β1,,]醛固酮;,肾小球系膜细胞;,纤维连接蛋白;,转化生长因子β1,1材料和方法,2结果,3讨论,参考文献:
Effects of aldosterone on synthesis of fibronectin and expression of transforming growth factorβ1 mRNA in cultured rat mesangial cellsLI Xiaodong, CHEN Xiaowen, TANG Deshen, LIANG Dong, LIU Huafeng
Institute of Nephrology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China
[Abstract] AIM: To evaluate the effect of aldosterone (ALD) and spironolactone (SPI, one of the aldosterone receptor antagonists) on synthesis and secretion of fibronectin (FN) and expression of transforming growth factorβ1 (TGFβ1 ) mRNA in cultured rat mesangial cells. METHODS: (1)Mesangial cells were treated with medium containing different concentrations of ALD (10-11, 10-9, 10-7 mol/L) and/or 10-7 mol/L SPI for 48 h, while control cells were treated with vehicle only. The levels of FN in the supernatants were measured by ELISA method. The expressions of FN mRNA and TGFβ1 mRNA were detected by semiquantitative RTPCR. (2)Mesangial cells were treated with 10-9 mol/L ALD for 24, 48, 72 h. The levels of FN in the supernatants were measured by ELISA method. The expressions of FN mRNA and TGFβ1 mRNA were detected by semiquantitative RTPCR. RESULTS: ELISA method showed that the level of FN in the supernatant of cultured rat mesangial cells stimulated with ALD increased significantly in a dose and timedependent manner. Also the expressions of FN mRNA and TGFβ1 mRNA were increased significantly by ALD in a dose and timedependent manner by semiquantitative RTPCR. SPI inhibited the stimulating effect of ALD on synthesis of FN and expressions of FN mRNA and TGFβ1 mRNA in cultured rat mesangial cells. CONCLUSION: ALD upregulates the protein synthesis and mRNA expression of FN, and upregulates the mRNA expression of TGFβ1 in cultured rat mesangial cells. SPI inhibits the effect of ALD, and thus implication in the treatment of Glomerulosclerosis. ......
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