41BB;_3H3;_CD8+_T细胞;

激动型抗41BB抗体3H3交联促进CD8+T细胞中Akt激酶活性

http://www.100md.com 《细胞与分子免疫学杂志》 2007年第3期

41BB;,3H3;,CD8+,T细胞;,Akt激酶,,]41BB;,3H3;,CD8+,T细胞;,Akt激酶,1材料和方法,2结果,3讨论,参考文献:

     Promotion of the activity of Akt in CD8+ T cells by the crosslink of 3H3, an agonist antibody of 41BB

    YANG Zhao, FU Xiaolan, SHANG Xiaoyun, NIU Wei, WU Yuzhang, WANG Li*

    Institute of Immunology, Third Military Medical University, Chongqing 400038, China

    [Abstract] AIM: To investigate whether 41BB could promote the proliferation and survival of CD8+ T cells proliferation, and the activity of protein kinase B (Akt). METHODS: highly purified CD8+ T cells were separated and stimulated by CD3 antibody. The crosslink of 3H3 and the proliferation and antiapoptosis of CD8+ T cells were examined. RESULTS: The crosslink of 3H3 promoted the proliferation and survival of CD8+ T cells. 41BB induced by 3H3 promoted the phosphorylated level and kinase activity of Akt. CONCLUSION: Akt pathway might participate in 41BB which promoted the proliferation and survival of CD8+ T cells induced by 3H3.

    [Keywords]41BB; 3H3; CD8+ T cell; Akt kinase

    [摘要] 目的: 研究41BB信号促进CD8+ T细胞增殖、存活及Akt活性的变化。方法: 利用小鼠CD8α+ T细胞磁珠负性分选试剂盒制备高纯度CD8+ T细胞, 并通过体外抗CD3单克隆抗体(mAb)刺激活化CD8+ T细胞。再通过流式细胞术(FCM)分析3H3交联与否, 四唑盐(WST1)/ECS检测CD8+ T细胞的增殖程度, Annexin V检测抗凋亡能力, 免疫印迹法分析胞内Akt磷酸化水平及Akt激酶活性 ......

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