Primary study of the recombinant immunotoxin DT390ª²mRantes in EAE therapy

    JIA Yi, LI Hong, CHEN Wenª²jie, LU Meiª²li, LI Mingª²yuan, JIANG Zhongª²hua, ZHANG Lin*

    Department of Material Evidence School of Preclinical and Forensic Medicine; National Key Laboratory of Biotherapy of Diseases, Sichuan University, Chengdu 610041, China

    [Abstract] AIM: To construct a novel eukaryotic expression plasmid including the recombinant immunotoxin DT390ª²mRantes and treat experimental autoimmune encephalomyelitis (EAE) in mice. METHODS: EAE in C57BL/6 mice were induced by the extracted MBP. The mRantes fragment was inserted into the eukaryotic expression plasmid SR¦Á containing DT390. Then cationic liposomeª²embedded plasmid DNA was injected into the muscles of the hindª²limbs in mice. The effect of DT390ª²mRantes was evaluated by observing clinical symptoms, pathological changes of brain, relative cytokine of peripheral blood, and the proportion of T cells and B cells. RESULTS: The recombinant immunotoxin DT390ª²mRantes was successfully constructed. Compared the mice in treated group with those in untreated group the clinical symptoms of EAE were alleviated, the infiltration of inflammatory cells were decreased, the IFNª²¦Ã level was fallen, and the ratio of T/B cells was decreased. CONCLUSION: The recombinant immunotoxin DT390ª²mRantes has distinct effects on EAE in mice, which may be used for beneficial reference to the therapy of MS. ......