Effects of Dengue virus infection on PGIS expression and PGI2 secretion of human vascular endothelial cells

    JIANG Zhenª²you1, XIAO Rui1, 2

    Department of Microbiology and Immunology, Medical College of Jinan University, Guangzhou 510632, China

    \[Abstract\] AIM: To elucidate the effects of Dengue virus on the expression and secretion of prostacyclin (PGI2) in endothelial cells. METHODS: Cultured human umbilical vein endothelial cells (HUVEC) were infected by Dengue virus II(DV2)for different duration. PGI2 level in the supernatant was determined by radioimmune assay. Cytoplasmic RNA of the infected HUVEC was prepared using the Trizol method and was assayed for prostacyclin synthase (PGIS)by RTª²PCR. RESULTS: PGIS expression as well as PGI2 secretion of the DV2 group were significantly increased (P<0.05) at 48 h, 72 h and 96 h postª²infection compared to the control group. CONCLUSION: DV2 could promote the expression of PGIS mRNA in HUVEC and increase the level of PGI2, which may increase the vascular permeability. The dysfunction of vascular endothelial cell (EC) induced by DV may be related to the pathogenesis of Dengue haemorrhagic fever (DHF)and Dengue shock syndrome(DSS). ......