Induction and mechanism study of antiª²melanoma immune response by M.tuberculosis Ag85B

    WU Xueª²zhong, WEI Haiª²ming*, ZHENG Xiaoª²dong, JIN Tengª²chuan, TIAN Zhiª²gang*

    Shandong Provincial Institute of Dermatology and Venereology, Jinan 250022,China

    \[Abstract\] AIM: To study the antiª²melanoma immunity efficacy of Ag85B antigen gene therapy in vivo. METHODS: C57BL/6 mice were inoculated s.c. with B16 cells, and 8 days later the mice were inoculated s.c. again with B16 cells (control group 1), B16/pcDNA3 cells (control group 2) or B16/pcDNA3ª²Ag85B cells (experimental group), respectively. Tumor volume, survival time, serum IFNª²¦Ã level and ILª²4 level of 3 groups mice were observed. Antitumor activity of Ag85B was studied. RESULTS: From 12 to 23 day, the mean tumor volume of mice increased from 1.1058 cm3 and 0ª±9123 cm3 to 7ª±5983 cm3 and 5.8746 cm3 in the control group 1 and 2, respectively. But it increased from 0ª±5158 cm3 to 1ª±5080 cm3 in the experimental group. The mean survival time of mice was 24ª±1 days and 24ª±7 days in the control group 1 and 2, respectively. That was 27ª±8 days in the experimental group. Within 13 days after the last inoculation, the serum IFNª²¦Ã level of all groups experienced increased (That increased to26ª±3ng/L,23ª±0ng/L and 25.2ng/L in the control group1,2 and the experimental group, respectively). Subsequently, the serum IFNª²¦Ã level in the two control groups decreased (That decreased to 19ª±3 ng/L and 18ª±3 ng/L in the control group 1 and 2) while it still augmented in the experimental group(That increased to 46ª±5 ng/L). ILª²4 level was slightly but not significantly enhanced and then declined in all mice.CONCLUSION: Ag85B induced the increase of serum IFNª²¦Ã level in the animals experiments, inhibited the tumor growth and prolonged the survival of the tumorª²bearing mice. ......