Effects of bone marrow stem cell transplantation at different time on heart function of rats with myocardial infarction

    HOU Zhiª²Feng1£¬ GONG Weiª²Qin1£¬ZHANG Shanª²Hong1£¬WANG Ying1£¬ WANG Yueª²Min2£¬LI Yuan1

    1Department of Geriatrics£¬ Xijing Hospital£¬ 2Department of Physiology£¬ School of Basic Medicine£¬ Fourth Military Medical University£¬ Xi¬ðan 710033£¬ China

    ¡¾Abstract¡¿ AIM: To investigate the optimal time for bone marrow mesenchymal stem cells (MSCs) transplantation after myocardial infarction (MI). METHODS: MI was induced by occluding the left anterior descending artery (LAD) in SD rats. MSCs were isolated from the rats, purified, proliferated and they (1 mL) were transfused through caudal vein into transplantation groups (n=10/group) at 1 d, 1, 2, and 4 weeks after MI; the same culture medium (1 mL) was transfused into the control group (n=10). Four weeks later, the heart function and myocardial ischemic size and infarct size were evaluated. RESULTS: The heart function and myocardial ischemic size and infarct size of 1ª²day group after MI had no difference from those of the control group (P>0.05) . The improvement of heart function in 1, 2, and 4ª²week groups were more distinct than that of 1ª²day group (P<0.05); myocardial ischemic size and infarct size were also smaller than those of 1ª²day group (P<0.05). Among the transplantation groups, the treatment effect of 2ª²weeks group was the best. CONCLUSION: Subacute stage (1-2 weeks after MI) might be the optimal time for MSCs transplantation. ......