胰腺炎_急性坏死性；肺损伤；细胞因子；谷胱甘肽；治疗

还原性谷胱甘肽对急性坏死性胰腺炎肺损伤的影响

http://www.100md.com 《第四军医大学学报》 2000年第5期

胰腺炎,急性坏死性；肺损伤；细胞因子；谷胱甘肽；治疗,,胰腺炎,急性坏死性；肺损伤；细胞因子；谷胱甘肽；治疗,【关键词】胰腺炎,急性坏死性；肺损伤；细胞因子；谷胱甘肽；治疗,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Effect of reduced glutathione on lung injury in rats with acute necrotizing pancreatitis

    PAN Deng1，JIA BaoQuan2, CAO Nong2

    1Clinical Medical College, 2Department of General Surgery, First Hospital, Lanzhou University, Lanzhou 730000, China

    【Abstract】 AIM: To investigate the changes of transcription factor nuclear factorκB (NFκB), TNFα, IL1β, IL18, myeloperoxidase (MPO) in the lung of rats with acute necrotizing pancreatitis (ANP) and explore the effects of reduced glutathione (GSH) on lung injury. METHODS: Wistar rats (n=72) were divided into 3 groups at random, ANP group, GSH treated group, and sham operation (SO) group. Animals in the 3 groups were killed 6, 12 and 18 h after operations and lung tissue, pancreas tissue were harvested. The expressions of IL18, IL1β, TNFα in lung tissue were measured by immunohistochemistry. MPO was determined by chromatometry. Intrapulmonary expression of NFkBp65 mRNA was detected by RTPCR. The histopathology of lung and pancreas was observed under the light microscope. RESULTS: The expressions of NFκB mRNA, IL18, IL1β, TNFα and the activity of MPO in lung tissue were much higher at 6, 12, 18 h in ANP group, with statistical significance compared with the SO group (P<0.05) and the levels of NFκB mRNA, IL1β and TNFα reached the peak at 12 h. After treatment with GSH, the levels of IL18, IL1β, TNFα became lower at 6, 12, 18 h and NFκB mRNA, MPO were decreased at 12 h (P<0.05 vs ANP group). CONCLUSION: All of NFκB ......

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