Effect of daqingye on EP3 mRNA expression of hypothalamus in ILª²1¦Âª²induced rabbits

    DONGJun1,2*,QIU Sheng1, XIE Xinª²hua1,PANRui3, TANG Hongª²mei1, LU Daª²xiang1, 2

    1Department of Pathophysiology, 2Key Lab of State Administration of Traditional Chinese Medicine, Medical College of Jinan University, Guangzhou 510632; 3Department of Orthopaedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China

    \[Abstract\] AIM: To study the antipyretic effect and mechanism of Daqingye injection (DQYI). METHODS: The ILª²1¦Âª²induced febrile New Zealand rabbits were chosen as experimental models and the antipyretic effect of DQYI was observed. The expression of EP3 mRNA was investigated by using in situ hybridization (ISH) in POAH. RESULTS: First, the colonic temperature went up gradually after intravenous(i.v) ILª²1¦Â. The peak value of temperature(¡÷T) and thermal response index (TRI1) in ILª²1¦Âª²treated group were higher than those in control group (P<0.01). The ¡÷T and TRI1 of in DQYI and ILª²1¦Âª² treated group were lower than those in ILª²1¦Âª²treated group (P<0.05). The temperature of DQYIª²treated group showed no distinguished difference compared with that in control group (P>0.05). Second, the expression of EP3 mRNA in the POAH of ILª² 1¦Âª²treated group increased markedly compared with that in control group (P<0.01). The expression of EP3 mRNA treated by ILª²1¦Â+DQYI in the POAH decreased strikingly compared with that in ILª²1¦Â group(P<0.05). CONCLUSION: DQYI has distinct antipyretic effect on ILª²1¦Âª²induced fever. The mechanism might be the inhibition of EP3 mRNA expression in POAH from rabbits. ......